MCP-1在慢性血栓栓塞性肺动脉高压大鼠肺组织中的表达及意义  

Expression of MCP-1 in pulmonary arterioles of rat with chronic thromboembolism pulmonary hypertension

作　　者：王佐岩[1] 张一梅[1] 刘温娟[1] 陈铭新[1] 鹿翠香[1] 

机构地区：[1]哈尔滨医科大学第一临床医学院呼吸内科,黑龙江150001

出　　处：《齐齐哈尔医学院学报》2010年第20期3202-3205,共4页Journal of Qiqihar Medical University

摘　　要：目的观察单核细胞趋化蛋白-1(MCP-1)在慢性血栓栓塞性肺动脉高压大鼠肺小动脉表达的动态变化。方法雄性Wistar大鼠64只,随机分为8组,每组8只:初始对照组、栓塞3d组、1周组、2周组、4周组、8周组、12周组、终末对照组。采血制备血栓,颈静脉注入,2周后第2次栓塞,全程腹腔注射氨甲环酸。达实验设定日期后,测各组大鼠平均肺动脉压(mPAP)、肺动脉相对中膜厚度(PAMT)、管壁面积/管总面积(WA/TA),免疫组化检测MCP-1和增殖细胞核抗原(PCNA)表达。结果栓塞4周组至12周组大鼠随时间延长mPAP明显增高(均P<0.01);PAMT、WA/TA在4周后随时间延长显著增高(4周组P<0.05,8周、12周组P<0.01);栓塞后MCP-1免疫组化染色强度随时间延长出现增高趋势(3d组P<0.05,4周组至12周组P<0.01),PCNA免疫组化染色强度随时间延长出现增高趋势(4周组至12周组P<0.01)。相关分析表明,MCP-1及PCNA与mPAP及血管重构指标均呈正相关(均P<0.01);MCP-1与PCNA表达呈正相关(r=0.812,P<0.01)。结论 MCP-1及PCNA均可能参与慢性血栓栓塞性肺动脉高压、肺血管重构的病理生理过程,炎症反应是慢性肺动脉高压形成重要机制之一。Objective To investigate the monocyte chemoattractant protein-1（MCP-1）in patients with chronic thromboembolic pulmonary hypertension in rats and the expression of pulmonary artery dynamics.Methods 64Male Wistar rats were randomly divided into 8groups of eight：the initial control group,the embolization group 3d,1week group,2weeks,4weeks,8weeks,12weeks,terminals in the control group.Preparation of blood clots,jugular vein injection,2weeks after the first 2embolism,the whole abdominal cavity injection of tranexamic acid.Experimental set up after the date of the rats measured mean pulmonary arterial pressure（mPAP）,pulmonary artery relative medial thickness（PAMT）,wall area/total area（WA/TA）,immunohistochemical detection of MCP-1and proliferation cell nuclear antigen（PCNA）expression.Results After 4weeks to 12weeks group mPAP increased significantly with time（all P0.01）;PAMT,WA/TA at 4weeks was significantly increased with time（4weeks group P0.05,8weeks,12weeks group P0.01）;embolization MCP-1immunohistochemical staining intensity increased with time trend appears（3dgroup group P0.05,4week group to 12weeks group P0.01）,PCNA immunohistochemical staining intensity increased with time trend appears（4weeks to 12weeks group P0.01）.Correlation analysis showed that,MCP-1and PCNA with mPAP and remodeling index were positively correlated（all P0.01）;MCP-1and PCNA expression was positively correlated（r=0.812,P0.01）.Conclusions MCP-1and PCNA are likely to participate in chronic thromboembolic pulmonary hypertension,pulmonary vascular remodeling in the pathophysiological process of chronic inflammation is an important mechanism of pulmonary hypertension.

关 键 词：血栓栓塞 肺动脉高压 单核细胞趋化蛋白-1 增殖细胞核抗原 

分 类 号：R543.2[医药卫生—心血管疾病]