X线修复交叉互补基因1多态性与1,3-丁二烯致接触工人染色体损伤易感性关系的研究  

Genetic polymorphism of XRCC1 associated with susceptibility of chromosomal damage in workers exposed by 1,3-butadiene

作　　者：王琪[1] 谭红汕[1] 王爱红[1,2] 冯楠楠[1] 叶云杰[1] 冯晓青[1] 夏昭林[1] 

机构地区：[1]复旦大学公共卫生学院劳动卫生教研室公共卫生安全教育部重点实验室,上海200032 [2]宁波市疾病预防控制中心

出　　处：《卫生研究》2010年第6期659-663,共5页Journal of Hygiene Research

基　　金：科技部“十一五”支撑计划项目(No.2006BAI06B02);国家自然科学基金项目(No.81072280);上海市公共卫生重点学科建设计划项目(No.08GW2X0402)

摘　　要：目的探讨DNA损伤修复基因X线修复交叉互补基因1(XRCC1)多态性在1,3-丁二烯致外周血淋巴细胞染色体损伤易感性中的作用。方法采用胞质分裂阻滞微核试验方法(CB-MN)评价166名1,3-丁二烯接触工人和41名正常人染色体损伤水平,应用聚合酶链反应-限制性片段多态性技术(PCR-RFLP)对接触工人第19染色体上的XRCC1第6外显子194密码子,第9外显子280密码子和第10外显子399密码子进行多态性检测。结果接触组和对照组的微核发生率分别为(3.39±2.42)‰和(1.48±1.26)‰,差异有显著性(P<0.01)。高剂量接触组工人比低剂量接触组工人更容易发生染色体损伤(FR=1.30,95%CI1.14～1.53,P<0.05)。XRCC1194突变基因携带个体的微核发生率比野生纯合型个体显著增高(FR=1.13,95%CI1.07～1.27,P<0.05);XRCC1280突变纯合型个体的微核率较野生纯合型显著增加(FR=1.67,95%CI1.10～2.42,P<0.05);XRCC1399杂合型个体以及杂合型和突变纯合型个体的微核率均显著增加(FR=1.26,95%CI1.03～1.53;FR=1.24,95%CI1.03～1.49,P<0.05)。CAG/TGG双体型个体微核率显著降低,结论 XRCC1194、280、399突变位点携带工人染色体损伤风险增高。Objective To explore the relationship between genetic polymorphism of XRCC1 194,280,399 and susceptibility of chromosomal damage induced by 1,3-butadiene（BD）.Methods 138 workers occupationally exposed to BD and 41 normal individuals were involved.Cytokinesis-block micronucleus（CB-MN） assay was used to detect chromosome damage in peripheral lymphocyte.PCR-RFLP technique was applied to detect polymorphisms in XRCC1 194,280 and 399.Results The MN frequencies（3.39 ± 2.42）‰ of workers exposed to BD were more higher than those of the non-exposed groups（1.48 ± 1.26）‰（P 0.01）.Workers receiving mere exposures had higher MN values than those of lower-exposed workers：frequency ratios（FR） = 1.30（95% CI 1.14-1.53,P 0.05）.Workers with XRCC1 194 Arg /Trp genotype had more susceptibility for chromosome damage,FR = 1.13（95% CI 1.07-1.27）.Workers with XRCC1 280 Arg/His genotype had more susceptibility for chromosome damage,FR = 1.67（95% CI 1.10-2.42）.XRCC1 399 Arg /Gln and combined group of Arg /Gln and Gln /Gln genotype carrier had higher MN frequency,FR = 1.26（95% CI 1.03-1.53） and 1.24（95% CI 1.03-1.49） respectively.The haplotypes CAG /TGG could associate with the decreased frequencies of total micronucleus（P 0.05）.Conclusion Genotype of XRCC1 could associated with the chromosome damage induced by BD.

关 键 词：1 3-丁二烯 XRCC1 基因多态性 职业接触工人 染色体损伤 职业卫生 

分 类 号：R595.2[医药卫生—内科学]