玉米赤霉醇染毒对雄性小鼠睾丸及生精功能的影响  

Effect of zeranol on spermary and spermigenesis function of male mice

作　　者：张振玲[1] 薄存香[1] 郭启明[1] 谢琳[1] 刘衍忠[1] 李莉[1] 

机构地区：[1]山东省职业卫生与职业病防治研究院,济南250062

出　　处：《卫生研究》2010年第6期755-758,共4页Journal of Hygiene Research

摘　　要：目的研究玉米赤霉醇对小鼠生精功能的影响及可能机制。方法选择健康性成熟的雄性昆明种小鼠40只,随机分为对照组和染毒组(玉米赤霉醇25、50、100mg/kg),连续灌胃染毒35天后脱臼处死。检测睾丸、附睾、精囊腺重量及脏器系数,附睾尾精子数、精子的形态结构及活力,并观察睾丸组织病理学改变。结果玉米赤霉醇各染毒组小鼠的精囊腺系数及精子计数均低于对照组,差异有显著性(P<0.05);50、100mg/kg组精子活动率下降,与对照组比较差异有显著性(P<0.05);睾丸组织有明显的病理改变,生精上皮细胞排列疏松、紊乱,层次减少,成熟精子数明显减少。结论在本实验剂量条件下,玉米赤霉醇对小鼠生精功能有毒性作用。Objective To study the effects of zeranol on spermiogenesis function of mice and its possible mechanism.Methods 40 healthy mature male mice were randomly divided into four groups：negative group and zeranol exposure groups（25,50,100mg /kg）.Male mice were respectively administered with zeranol at the doses of 25,50,and 100mg /kg for 35 days and killed.The weights of spermary,epididymis and spermatocyst were measured.The sperm counting,motility and the percent of abnormal sperms were observed.Pathological changes of testicle tissue were also observed.Results In comparision with control group,the visceral coefficient of spermatocysts and sperm counting of zeranol exposure groups were all decreased（P 0.05）.Sperm motility were significantly decreased in the median and high exposure groups（P 0.05）.Pathological alterations of testes were also observed.Seminiferous epithelium was reduced in exposure groups,loose and anomalistic organization.Sperm counts were also reduced in the lumina of seminiferous tubules with glair.Conclusion Zeranol have toxicity effect on spermigenesis function of male mice.

关 键 词：玉米赤霉醇 雄性小鼠 睾丸 生精功能 染毒 激素 

分 类 号：Q579.13[生物学—生物化学] Q132.7