烟草Ⅰ类几丁质酶和β-1,3-葡聚糖酶cDNA植物双价表达载体的构建及转化番茄的研究  

Construction of Plant Bivalent Expression Vector of Tobacco Ⅰ-Chitinase and b-1, 3-Glucanase cDNA and Its Transformation into Tomato

作　　者：李继红[1] 邵寒霜[1] 郑楷[1] 胡东琼[1] 郑学勤[1] 

机构地区：[1]中国热带农业科学院热带作物生物技术国家重点实验室,海口571101

出　　处：《热带作物学报》1999年第1期29-33,共5页Chinese Journal of Tropical Crops

摘　　要：将烟草中经修饰的Ⅰ类几了质酶和β－1，3-葡聚糖酶cDNA分别加上CaMV35S启动子和NOS终止子，依次插入到同一植物表达载体pBin19上，获得植物双价表达载体pCG－Ⅱ。然后载体pCG－Ⅱ以土壤农杆菌LBA4404介导转化番茄，再生植株的点杂交及PCR扩增证明两种cDNA已随T－DNA同时导入番茄植株中。The modified tobacco Ⅰ-Chitinase (Chi) and b-1, 3 -Glucanase (Glu) cDNAwith CaMV 35 S promotor and NOS terminator were cloned into pBin19, and a plantbivalent expression vector pCG-Ⅱ was obtained. The pCG-Ⅱ was then transformed intotomato through mediation of Agrobacterium tunefaciens LBA4404. The dot-blot hybridization and PCR amplification analysis demonstrated that the Chi and Glu cDNA were integrated into tomato genomes.

关 键 词：几丁质酶 CDNA β-1 3-葡聚糖酶 CDNA 载体构建 

分 类 号：S641.2[农业科学—蔬菜学] Q78[农业科学—园艺学]