蜀葵简约化快繁模式的建立  

Tissue culture and rapid propagation system of Hollyhock

作　　者：王洪军 卢宝伟 安凤霞[2] 

机构地区：[1]黑龙江省生态工程职业学院 [2]黑龙江省科学院自然与生态研究所,哈尔滨150040

出　　处：《国土与自然资源研究》2010年第4期74-75,共2页Territory & Natural Resources Study

基　　金：黑龙江省教育厅高职高专院校科学技术研究项目蜀葵组织培养及工厂化生产技术研究课题编号:11525080

摘　　要：本文基于生产需要,改进蜀葵组培快繁体系,建立一套简约化快繁模式,用蜀葵无菌苗的茎段做为外植体对蜀葵进行离体快速繁殖,在组培过程中简化培养基和培养条件,茎段接种4～5周直接诱导出不定芽,芽诱导培养基MS+6-BA1mg/L为最佳;增殖培养以MS+6-BA2mg/L+NAA1mg/L为好,其增殖系数较高,幼苗生长快而健壮;而根的诱导则以1/2MS+蔗糖浓度10g/L相对理想。The demand for the Hollyhock is gradually growing in recent years. So it＇s necessary to establish the reduction and the rapid propagation system of tissue culture. In this paper, stem segments of axenic seedlings of hollyhock are the explants for rapid propagation in vitro. Adventitious buds are induced directly after stem segments inoculated 4 to 5 weeks. The best inductive conditioned medium of bud is MS ＋ 6- BA1mg / L; Nutrient fluid of value cultivating, MS ＋6- BA2mg / L ＋ NAA0.1mg / L , has high multiplication coefficient and can make seedlings grow fast and robustly. To root induction, the prescription as 1/2MS ＋10g / L sucrose is relatively good.

关 键 词：蜀葵 简约化 快繁 

分 类 号：Q945.5[生物学—植物学]