胶原蛋白-壳聚糖支架的构建及其与骨髓基质干细胞复合的实验研究  

Development of Collagen-chitosan Scaffold and Its Combination with Bone Marrow Stem Cells

作　　者：尤凤健[1] 胡学昱[1] 罗卓荆[1] 

机构地区：[1]第四军医大学西京医院全军骨科研究所,陕西西安710032

出　　处：《现代生物医学进展》2010年第21期4012-4014,共3页Progress in Modern Biomedicine

基　　金：国家自然科学基金(No.30770571)

摘　　要：目的:构建一种组织工程神经支架,并观察体外培养的骨髓基质干细胞在其内部的生长情况,为后续种子细胞的移植提供阶段性实验数据。方法:以Ⅰ型胶原蛋白和壳聚糖为原料通过冷冻干燥技术制备神经支架,扫描电镜观察其内部结构,测量其孔径大小、孔隙率等指标。将体外培养的骨髓基质干细胞与Ⅰ型胶原蛋白-壳聚糖神经支架复合,共培养2天;扫描电镜观察细胞在支架内部的生长情况。结果:构建的神经支架均为圆柱状,内部为纵向平行排列的孔径均匀的微管样结构,细胞紧密贴附在支架微孔内壁上,细胞生长状况良好。结论:Ⅰ型胶原蛋白-壳聚糖支架具有良好的内部三维结构和生物相容性,可与细胞复合后用于修复周围神经缺损。Objective：To develop a tissue engineering nerve scaffold,and observe the growth of bone marrow stem cells cultured in vitro on the scaffold,to provide the stage-experiment data for the transplantation of follow-up seed cells.Methods：The biomaterial was made of I-collagen and chitosan by freeze-drying method,the alignment regularities of microscopic channels and there course directions were observed under the scanning electronic microscope.The size of the micropores and the factor of porosity were also measured.The bone marrow stem cells were seeded on the collagen scaffolds and cultured for 3 days,and then the growth of bone marrow stem cells cultured in the scaffold was observed.Results：All the scaffolds were circular cylinder,the microscopic channels were arranged in parallel manners,and the pore sizes of the channels were uniform.The bone marrow stem cells were seeded in the scaffolds successfully.Conclusion：I-collagen-chitosan scaffolds have good structure and biocompatibility,which can be used for repairing the nerve injuries after combined with bone marrow stem cells.

关 键 词：组织工程 骨髓基质干细胞 Ⅰ型胶原蛋白 壳聚糖 

分 类 号：R318[医药卫生—生物医学工程] R687[医药卫生—基础医学]