北京地区儿童携带的酿脓链球菌emm分型和超抗原speA及speC的相关性研究  

Relationship between emm Typing and Superantigen Genes speA and speC of Streptococcus Pyogenes Isolated from Children in Beijing

作　　者：梁云梅[1,2] 常贺生[1,3] 沈叙庄[1] 俞桑洁[1] 袁林[1] 杨永弘[1] 

机构地区：[1]首都医科大学附属北京儿童医院微生物室,北京100045 [2]北京世纪坛医院儿科,北京100038 [3]首都医科大学附属北京朝阳医院儿科,北京100020

出　　处：《实用儿科临床杂志》2010年第22期1700-1702,共3页Journal of Applied Clinical Pediatrics

基　　金：国家863计划(2006AA02z417)

摘　　要：目的分析北京地区儿童携带的酿脓链球菌酿脓链球菌的emm分型及携带超抗原speA及speC基因情况。方法应用PCR对2007年1-12月155株北京地区猩红热、咽扁桃体炎患儿及健康儿童咽部酿脓链球菌emm基因及超抗原speA和speC基因进行扩增。结果健康儿童、猩红热患儿及咽扁桃体炎患儿中有emm1.0(51.6%)、emm4.0(0.6%)、emm12.0(41.3%)、emm18.0(1.9%)、emm22.0(0.6%)、st1815(1.3%)、st5240(0.6%)及stG485(1.9%)8种酿脓链球菌克隆株流行。其中13.5%酿脓链球菌只携带超抗原speA基因,26.5%只携带超抗原speC基因,40.6%同时携带超抗原speA和speC基因,19.4%2种基因均不携带。25.0%emm1.0型酿脓链球菌只携带超抗原speA基因,2.5%emm1.0型和51.6%emm12.0型酿脓链球菌只携带超抗原speC基因,68.8%emm1.0型和12.5%emm12.0型酿脓链球菌同时携带speA及speC基因,3.8%emm1.0型和35.9%emm12.0型酿脓链球菌2种基因均不携带。27.3%致猩红热emm1.0型酿脓链球菌只携带超抗原speA基因,4.5%只携带超抗原speC基因,61.4%同时携带超抗原speA和speC基因,6.8%克隆株2种基因均不携带。18.8%致咽扁桃体炎emm1.0型酿脓链球菌只携带超抗原speA基因,81.2%emm 1.0型酿脓链球菌同时携带超抗原speA和speC基因。50.0%健康儿童携带的emm1.0型酿脓链球菌只携带超抗原speA基因,50.0%同时携带超抗原speA和speC基因。39.1%致猩红热emm12.0型酿脓链球菌只携带超抗原speC基因,17.4%同时携带超抗原speA和speC基因,43.5%2种基因均不携带。73.3%致咽扁桃体炎emm12.0型酿脓链球菌只携带超抗原speC基因,10.0%同时携带超抗原speA和speC基因,16.7%2种基因均不携带。18.2%健康儿童携带的emm12.0型酿脓链球菌只携带超抗原speC基因,9.1%同时携带超抗原speA和speC基因,72.7%2种基因均不携带。结论 emm1.0型和emm12.0型酿脓链球菌是导致北京地区儿童发生猩红热及咽扁桃体炎的2个主要克隆菌株。stG485、emm18.0、emm1.0型和emm12.0型酿Objective To analyze the emm typing and superantigen genes（speA and speC） of streptococcus pyogenes isolated from children in Beijing with scarlet fever or pharyngitis,or who were asymptomatic carriers.Methods One hundred and fifty-five streptococcus pyogenes were isolated from children with scarlet fever,adenopharyngitis or healthy carriers in Beijing from Jan.to Dec.2007.Their superantigen genes（speA and speC） and en-coding mature M protein genes（emm gene） were amplified by PCR.Results Eight emm types were discovered： emm1.0（51.6%）,emm4.0（0.6%）,emm12.0（41.3%）,emm18.0（1.9%）,emm22.0（0.6%）,st1815（1.3%）,st5240（0.6%） and stG485（1.9%）.Of these emm types,13.5% carried the superantigen speA,26.5% carried speC,40.6% carried both speA and speC,and 19.4% carried neither speA nor speC.Twenty-five percent of the emm1.0 isolates carried speA,2.5% emm1.0 and 51.6% emm12.0 isolates carried speC,68.8% emm1.0 and 12.5% emm12.0 isolates carried both speA and speC,3.8% emm1.0 and 35.9% emm12.0 isolates carried neither speA nor speC.Among the emm1.0 isolates with scarlet fever,27.3% carried the superantigen speA,4.5% carried speC,61.4% carried both speA and speC,and 6.8% carried neither speA nor speC.Among the emm1.0 isolates with adenopharyngitis,18.8% carried the superantigen speA,81.2% carried both speA and speC.Among the emm1.0 asymptomatic carrier isolates,50.0% carried the superantigen speA,50.0% carried both speA and speC.Among the emm12.0 isolates with scarlet fever,39.1% carried speC,17.4% carried both speA and speC,and 43.5% carried neither speA nor speC.Among the emm12.0 isolates with adenopharyngitis,73.3% carried speC,10.0% carried both speA and speC,and 16.7% carried neither speA nor speC.Among the emm12.0 asymptomatic carrier isolates,18.2% carried speC,9.1% carried both speA and speC,and 72.7% carried neither speA nor speC.Conclusions The common emm types causing scarlet fever or adenopharyngitis are emm1.0 and emm12.0.The common asymptomatic carrier emm types are emm12.

关 键 词：猩红热 咽扁桃体炎 健康携带者 酿脓链球菌 超抗原 emm分型 

分 类 号：R725.1[医药卫生—儿科]