L-ficolin基因Ala258Ser位点多态性与反复呼吸道感染的关系  

Association of L-Ficolin Gene Ala258Ser Polymorphism and Recurrent Respiratory Tract Infection in Children

作　　者：王劲波[1] 王永清[1] 罗光华[2] 张俊[2] 牟琴峰[2] 杨晓宇[3] 

机构地区：[1]苏州大学附属第三医院儿科,江苏常州213003 [2]苏州大学附属第三医院综合实验室,江苏常州213003 [3]苏州大学附属第三医院心内科,江苏常州213003

出　　处：《实用儿科临床杂志》2010年第22期1715-1716,1722,共3页Journal of Applied Clinical Pediatrics

基　　金：常州市卫生局重大招标课题(ZD200709)

摘　　要：目的探讨江苏地区汉族儿童L-ficolin基因(FCN2)第8外显子A la258Ser位点多态性与反复呼吸道感染(RRTI)的发病是否相关。方法选取2008年2月-2009年2月在本院儿科就诊的常州市患儿318例。经详细问卷调查,根据患儿既往患病情况将其分为RRTI组(104例)及对照组(214例)。RRTI诊断标准采用2007年中华医学会儿科学分会呼吸学组制定的RRTI的诊断标准。由上海申能博彩生物科技有限公司提供试剂。在罗氏公司LightCycler基因扩增检测仪上通过PCR进行目的 DNA扩增,采用碱基淬灭探针技术检测FCN2第8外显子A la258Ser位点单核苷酸多态性。采用基因计数法及2χ检验等统计学方法分析二组儿童A la258Ser位点基因型及等位基因频率分布是否存在差异。结果二组患儿性别、年龄比较差异无统计学意义。A la258Ser位点基因型分布:野生纯合子GG基因型在RRTI组和对照组中分别为63.5%和66.4%,TG杂合子基因型分别为31.7%和31.3%,突变纯合子TT基因型分别为4.8%和2.3%,二组基因型分布比较差异无统计学意义(2χ=1.45,P=0.48);野生型G等位基因频率在RRTI组和对照组中分别为79.3%和82.0%,突变型T等位基因频率在二组中分别为20.7%和18.0%,二组比较差异均无统计学意义(2χ=0.66,P=0.42)。结论 L-ficolin基因第8外显子A la258Ser位点基因多态性与江苏地区汉族儿童RRTI无关。Objective To explore the association between polymorphisms of the Ala258Ser in the FCN2 and the incidence of recurrent respiratory tract infection（RRTI） in Chinese Han children in Jiangsu area.Methods Three hundred and eighteen children in Changzhou who were in hospital from Feb.2008 to Feb.2009 were selected,and they were divided into RRTI group（104 cases） and control group（214 cases） according to the parental questionnaires.Diagnostic criteria according to the diagnostic criteria approved by the Chinese Academy of Pediatrics in 2007 on RRTI.Reagents were obtained from Shenergy Biocolor（Shanghai,China） and thermal cycling was performed in a LightCycler（Roche）.The genotypes of Ala258Ser polymorphisms in the FCN2 were detected by polymerase chain reaction and base-quenched probe technique in RRTI children and controls.Significance was calculated using gene counting and Chi-Square test.Results Patients in 2 groups were matched for sex and age.Ala258 Ser genotype distribution in RRTI group were as follows：GG was 63.5%,TG was 31.7% and TT was 4.8%.In control group,GG,TG and TT genotype were 66.4%,31.3% and 2.3%,respectively;there was no significant difference between RRTI group and control group（χ2=1.45,P=0.48）.The allele frequencies of T in the 2 groups were 20.7% and 18.0%,respectively;the allele frequencies of G in the 2 groups were 79.3% and 82.0%,respectively;there was no significant difference between the 2 groups（χ2=0.66,P=0.42）.Conclusion The polymorphisms of Ala258Ser in FCN2 gene exon 8 do not seem to contribute to the prediction of RRTI in Chinese Han children in Jiangsu.

关 键 词：反复呼吸道感染 L-FICOLIN FCN2 多态性 单核苷酸 

分 类 号：R725.6[医药卫生—儿科]