灵芝中灵芝酮二醇的分离鉴定及抗肿瘤活性  

Separation and identification of ganodermanondiol and its anti-proliferative effect on tumor cells

作　　者：李鹏[1] 魏晓霞[1] 郭晓丹[1] 吴敏[1] 许建华[1] 

机构地区：[1]福建医科大学药学院,福建福州350004

出　　处：《中国医院药学杂志》2010年第22期1889-1891,共3页Chinese Journal of Hospital Pharmacy

基　　金：福建省教育厅科学技术研究资助项目(编号:JA08104);福建医科大学苗圃科研基金项目(编号:2010MP044)

摘　　要：目的:从灵芝提取物中分离纯化灵芝酮二醇,并探讨其对8种肿瘤细胞的抑制作用。方法:灵芝氯仿提取部分经反复硅胶柱层析得到一个白色针晶,经熔点测定1、H-NMR和13C-NMR数据分析以及与已知化合物比较,确定其化学结构。利用MTT法检测其对人Burkitt淋巴瘤细胞株CA46、人胃癌细胞株SGC7901、人结肠癌细胞株SW1116、人结肠癌细胞株SW480、人肝癌细胞株SMMC7221、人肝癌细胞株HepG2、人髓系白血病细胞株HL60、人慢性粒细胞白血病细胞株K562的生长抑制情况。结果:经理化性质鉴定、波谱分析,确定白色针晶结构为24(S),25-二羟基-5α-羊毛甾-7,9(11)-二烯-3-酮,即灵芝酮二醇。MTT结果表明,灵芝酮二醇对HL60和K562 2种瘤株有较强抑制作用,对CA46、SMMC7221、HepG2、SW480、SW1116及SH-SY5Y6种瘤株抑制作用较弱。结论:灵芝三萜类成分灵芝酮二醇对人白血病肿瘤细胞HL60及K562的增殖有一定的抑制作用,初步确定灵芝酮二醇为灵芝抗白血病的活性成分之一。OBJECTIVE To isolate ganodermanondiol from Ganoderma lucidum, and to investigate its anti-proliferative effect on the growth of eight kinds of tumor cells. METHODS A compound was isolated by the silica gel column chromatogra phy from chloroform extract of Ganoderma lucidum. Its chemical structure was established on the basis of physical and chemical properties, and 1H-NMR and 13 C-NMR spectroscopy. The cytotoxicity of compound against eight kinds of cancer cell lines was evaluated by MTT assay. RESULTS The chemical structure of the compound was determined as 24（S）-25-dihydroxy 5mlanos- ta-7,9（ 11 ） dien 3-one（ganodermanondiol）. The results of MTT assay showed that ganodermanondiol had an inhibitory effect on the proliferative of HL60 and K562 human tumor cells in a concentration dependent manner. But ganodermanondiol had less inhibitory activity on CA46,SMMC7221,HepG2,SW480,SW1116 and SH SY5Y human tumor cells. CONCLUSION Ganoder manondiol can significantly inhibit the proliferation of leukemic cancer cells, and it could be one of the antileukemie active con stituents of Ganoderma lucidum.

关 键 词：灵芝酮二醇 灵芝 体外抗肿瘤作用 

分 类 号：R285.2[医药卫生—中药学]