(顺)-3-(氯代亚甲基)-6-甲基-硫色满-4-酮的体外抗肿瘤活性研究  

Antitumor activities of (Z)-3-(chloromethylene)-6-methylthiochroman-4-one in vitro

作　　者：李春纳[1] 杨春柳[1] 刘玉欣[1,2] 杨更亮[1,2] 刘腾飞[1] 路倩[1] 

机构地区：[1]河北大学药学院,保定071002 [2]河北省药物质量分析控制重点实验室,保定071002

出　　处：《科学通报》2010年第31期3027-3031,共5页Chinese Science Bulletin

基　　金：国家自然科学基金资助项目(20375010;20675084)

摘　　要：以生长抑制率为指标,采用改良的MTT法检测新合成的(顺)-3-(氯代亚甲基)-6-甲基-硫色满-4-酮(CMMT)对12株人源肿瘤细胞系(A549,SGC-7901,BGC-823,U937,K562,Hela,MCF-7,HEPG-2,A375,LS174T,HT1080,C4-2B)增殖的影响.CMMT对12种细胞的半数抑制浓度(IC50)在0.41～6.05μg·mL-1之间.与同浓度的顺铂(CDDP)相比,其抗肿瘤活性明显占优.CMMT能够明显抑制肿瘤细胞的生长,具有极高的抗肿瘤活性,研究结果为深入研究该类药物的体内抗肿瘤作用提供了有力的实验数据.Anti-proliferative activity of a novel synthesized thiochromanone derivative,（Z）-3-（chloromethylene）-6-methylthio-chroman-4-one （CMMT） was investigated in this paper.It was tested for anti-proliferative activity against the 12 human tumor cell lines （A549,SGC-7901,BGC-823,U937,K562,Hela,MCF-7,HEPG-2,A375,LS174T,HT1080 and C4-2B） by the MTT assay.The results showed that CMMT could exhibit dramatically antitumor activities at a low concentration （IC50 were 0.41？6.05 μg/mL）.Importantly,the inhibition effect of CMMT on 12 tumor cells were much higher than cisplatin at the same concentration （10.0 μg/mL）.Pressent studies indicated that CMMT could significantly inhibit cell proliferation,and provided preliminarily valuable data for further investigatory both in vitro and in vivo studies.

关 键 词：(顺)-3-(氯代亚甲基)-6-甲基-硫色满-4-酮 抗肿瘤活性 MTT法 增殖 半数抑制浓度 

分 类 号：R965[医药卫生—药理学]