利用树干毕赤酵母发酵玉米秸秆制备燃料酒精  

Fuel ethanol production from corn stover by Pichia stipitis

作　　者：张强[1] 戈兴炜[2] Anders Thygesen Anne Belinda Thomsen 

机构地区：[1]长春理工大学生命科学技术学院,吉林长春130022 [2]长春理工大学校医院,吉林长春130022 [3]Biosystems Division,Risφ DTU,Technical University of Denmark

出　　处：《化工进展》2010年第12期2270-2273,共4页Chemical Industry and Engineering Progress

基　　金：吉林省教育厅科技项目(200623);吉林省科技发展农业重点项目(20030203)资助

摘　　要：采用湿热预处理(195℃,15min)与同步糖化发酵对玉米秸秆制备酒精进行了研究。结果表明:玉米秸秆经过湿热预处理后,86.5%纤维素保留在滤饼中,而大部分半纤维素被溶解。在底物质量体积含量50g/L,温度30℃,pH值5.5,摇床转速130r/min条件下,树干毕赤酵母(Pichia stipitis)利用预处理后的玉米秸秆经过192h同步糖化发酵,酒精浓度达到了12.12g/L,对应的酒精产量和生产效率分别为0.34g/g(葡萄糖+木糖)和0.065g/(L·h)。该项研究为工业化生产打下了基础。Hydrothermal pretreatment（195 ℃,15 min）and simultaneous saccharification and fermentation（SSF）were adopted to produce ethanol from corn stover.Results showed that 86.5% of cellulose was remained in the solid cake and most of hemicellulose was solubilized after the hydrothermal pretreatment.The highest ethanol concentration of 12.12 g/ L was achieved at initial pH of 5.5,temperature of 30 ℃ and 130 r/min shaking rate with substrate concentration of 50 g/L by Pichia stipitis after 192 h.The corresponding alcohol yield and volumetric productivity were 0.34 g/g （glucose ＋ xylose）and 0.065 g/（L·h） respectively.

关 键 词：玉米秸秆 湿热预处理 同步糖化发酵 树干毕赤酵母 

分 类 号：TK6[动力工程及工程热物理—生物能]