电针不同介入时机对痛经模型大鼠子宫微循环影响的实验观察  

Experimental Study on the Uterus Microcirculation of Electro-Acupuncture(EA) on Dysmenorrhea Rat Model in Different Intervening time

作　　者：嵇波[1] 李春华[1] 赵雅芳[1] 任晓暄[1] 郭孟玮[1] 丁喜艳[1] 张露芬[1] 朱江[1] 

机构地区：[1]北京中医药大学针灸推拿学院,北京100029

出　　处：《针灸临床杂志》2010年第11期46-51,共6页Journal of Clinical Acupuncture and Moxibustion

基　　金：国家重点基础研究发展计划(973计划)课题;编号:2006CB504503

摘　　要：目的:比较电针三阴交穴不同介入时机对痛经模型大鼠子宫微循环的影响,探讨介入时机与针刺效应的关系。方法:将动情间期雌性大鼠随机分为6组,按照介入时机分为即刻电针组(A组)和预先电针组(B组),两组又分别分为盐水组、模型组、针刺组。除盐水组外,其余各组大鼠均连续10天给予皮下注射苯甲酸雌二醇注射液,末次给药1h后,腹腔注射缩宫素2u/只,制备痛经大鼠模型。盐水组每日给予同等剂量的生理盐水。针刺A组于第10日给予电针20min,针刺B组于第8日给予电针20min,盐水B组及模型B组束缚20min,每日1次,连续3天。于第10日采用XW-B-3冷光源微循环显微检查仪观察各组大鼠不同时间段子宫微循环的变化。结果:与模型A组比,即刻电针三阴交20min时,微血管、毛细血管(cap)管径明显扩张,cap条数明显增多,差异显著(P<0.01或P<0.05),电针10、20min时,微血管条数明显增多,差异显著(P<0.05),血流状态无显著性差异;与模型B组比,预先电针三阴交5、10、20min时微血管管径明显扩张,微血管条数明显增多,血流明显加快,差异显著(P<0.01或P<0.05),电针10、20min时,cap管径明显扩张,cap条数(除10min外)明显增多,差异显著(P<0.01或P<0.05)。结论:即刻、预先针刺三阴交穴均可缓解子宫血管的痉挛状态,改善子宫微循环,缓解疼痛,但预先针刺起效快、作用程度强,明显优于即刻针刺。Objective：To compare effects of uterus microcirculation of EA Sanyinjiao（SP6）on dysmenorrhea rat model in different intervening time,to discuss the relationship between intervening time and effects of acupuncture.Methods：Diestrus female rats were randomly divided into 6 groups,according to different intervening time rats were divided into immediate EA group（group A）and pre-EA group（group B）.Two groups were subdivided into saline group,model group,acupuncture group.Estradiol benzoate and oxytocin were chosen to be the model of dysmenorrhea.Except saline group,other groups were given subcutaneous estradiol benzoate injection,after the last administration 1h,intra-peritoneal injection of oxytocin 2u/each,Saline group was given the same dose of saline per day,acupuncture group A was given EA for 20mins on the 10th day,acupuncture group B was given EA for 20mins on the 8th day,saline group and model group were bounded for 20 mins,once a day,for 3 days.On the 10th day,use XW-B-3 Cold Light micro-circulation tester to observe the changes of uterine microcirculation at different time.Results：Compared with model group A,EA on SP6 for 20mins,diameters of micro-vessels and caps were significantly expanded,numbers of caps were significantly increased（P0.01、P0.05）,EA on SP6 for 10 mins、20 mins,numbers of micro-vessels were significantly increased（P0.05）,the state of blood flow hadn＇t significant difference（P0.05）;Compared with model group B,pre-EA on SP6 for 5mins、10mins、20mins,diameters of micro-vessels were significantly expanded,numbers of micro-vessels were significantly increased,the state of blood flow sped up significantly（P0.01、P0.05、P0.01、P0.05）,pre-EA on SP6 for 10mins、20mins,caps diameters were significantly expanded,Numbers of caps were significantly increased（except pre-EA 10mins）（P0.05、P0.01、P0.05）.Conclusion：Both EA and pre-EA can relieve spasm of uterus blood vessels,improve micro-circulation and relieve pain,but pre-EA is better than EA in work

关 键 词：痛经 子宫微循环 三阴交 即刻针刺 预先针刺 

分 类 号：R246.1[医药卫生—针灸推拿学]