杜鹃红山茶离体快速繁殖  

In Vitro Propagation of Camellia changii

作　　者：蔡静如[1,2] 庄雪影[1] 杨跃生[3] 尹茜[1] 翟翠花[1] 

机构地区：[1]华南农业大学林学院,广东广州510642 [2]广东省林业科学研究院,广东广州510520 [3]华南农业大学生命科学学院,广东广州510642

出　　处：《热带作物学报》2010年第9期1469-1473,共5页Chinese Journal of Tropical Crops

基　　金：广东省林业局科技项目资助

摘　　要：以杜鹃红山茶实生小苗为试验材料,探讨离体快速繁殖的方法。结果表明:茎段外植体在MS+BA 1 mg/L+IBA 0.01 mg/L+GA3 10 mg/L的培养基上培养60 d,侧芽萌发率高达68%;将这些萌发的侧芽连同原来的茎段外植体移至添加BA 0.2～0.4 mg/L的培养基培养1个月,74%的侧芽能够继续生长并形成丛生芽;再将丛生芽移至1/2 MS+IBA 4 mg/L+0.1%活性炭的培养基上培养1个月,得到大量伸长的芽条;将芽条切离母体后插植于1/2 MS+IBA 8 mg/L的培养基,60 d后生根率达到90%。通过以上过程繁殖得到的小苗质量良好,移栽1个月后的成活率可达90%。Seedlings were used as starting materials for investigation on in vitro propagation of Camellia changii.It was found that 68% axillary buds on the explants of stem segments of the seedlings germinated after culture on MS medium containing BA 1 mg/L,IBA 0.01 mg/L and GA3 10 mg/L for two months.When these buds with the mother tissues were subcultured on MS medium containing BA 0.20.4 mg/L for one month,74% of the germinated buds formed multiple tiny shoots.These shoots elongated prominently upon subculture on half strength MS medium containing IBA 4 mg/L and active charcoal 0.1% for one month.These elongated shoots,after isolating from the mother tissues,were transferred to half strength MS medium containing IBA 8 mg/L,a rooting rate as high as 90% were achieved in two months.The so far obtained plantlets were of rather good quality with a survival rate of 90% observed one month after transplantation.

关 键 词：杜鹃红山茶 离体培养 茎段外植体 生根 移栽 

分 类 号：S682.190[农业科学—观赏园艺]