巴西橡胶芽接树砧穗互作的蛋白质组初步研究  

The Preliminary Proteome Study on the Interaction between Rootstock and Scion of Budgrafted Rubber Tree

作　　者：丁璇[1,2,3] 袁坤[1,2] 杨礼富[1,2] 曹建华[1,2] 徐志娟[1,3] 何哲[1,3] 林位夫[1,2] 

机构地区：[1]中国热带农业科学院橡胶研究所,海南儋州571737 [2]农业部橡胶树生物学重点开放实验室,海南儋州571737 [3]海南大学农学院,海南儋州571737

出　　处：《热带作物学报》2010年第9期1514-1518,共5页Chinese Journal of Tropical Crops

基　　金：农业部公益性行业科研专项"橡胶芽接树优良砧木选择与快繁技术研究"(nyhyzx07-033-1-08);农业部科研院所基本业务费项目"橡胶树品种改良技术研究(3);橡胶芽接树优良砧木选择机理研究(一)"(ywf2007);天然橡胶产业技术体系;海南省自然科学基金项目"巴西橡胶树死皮病发生机制的比较蛋白质组学解析"(30819)资助

摘　　要：运用蛋白质组学研究技术,寻找砧木和接穗互作相关蛋白。以巴西橡胶树3种芽接组合植株的砧木与接穗结合部附近处的胶乳为试验材料,经双向凝胶电泳(2-DE)分离获得胶乳中存在的差异表达蛋白,采用质谱技术鉴定出32个差异表达蛋白,这些蛋白主要参与转录、翻译、光合作用、细胞分裂、蛋白质合成和胶乳合成等生物学过程。The proteomics research technique was applied to find the proteins related to the interaction between rootstock and scion.Latex colleced from the rootstock and scion parts of 3 budgrafted rubber trees as the experimental materials,differentially expressed proteins were obtained by 2-DE.Using MS technique,32 differentially expressed proteins were identified.These proteins were mainly involved in transcription,translation,photosynthesis,cell division,protein synthesis and latex synthesis.

关 键 词：橡胶树 砧木 接穗 相互作用 蛋白质组 双向电泳 质谱 

分 类 号：S794.1[农业科学—林木遗传育种]