尖吻蝮蛇蛇毒中纤溶组分与铽的相互作用  

Intraction between Tb^(3+) and Fibrinolytic Principle from Agkistrodon Acutus Venom

作　　者：王守业[1] 丁兰[2] 刘清亮[1] 赵大庆[2] 陈真龙[1] 张祖德[1] 

机构地区：[1]中国科学技术大学化学系,合肥230026 [2]中国科学院长春应用化学研究所稀土化学与物理开放实验室,长春130022

出　　处：《中国稀土学报》1999年第1期1-6,共6页Journal of the Chinese Society of Rare Earths

基　　金：国家自然科学基金!29471027;中国科学院长春应用化学所稀土化学与物理开放实验室基金

摘　　要：利用平衡透析、原子吸收、荧光滴定和荧光寿命测定等方法确定了皖南尖吻蝮蛇蛇毒纤溶组分（FP）中Ca（2+）含量，并利用荧光光谱研究了Tb（3+）与FP中色氨酸（Trp）残基之间能量转移和FP中的微区结构。结果表明，每个FP分子中只含有一个Ca(2+)；Tb(3+)可完全取代FP中的Ca(2+)，FP中的Trp残基可将能量转移到结合在FP中的Tb(3+)上；研究还测得Tb(3+)与FP中Trp残基之间的距离约为0．375nm。By using equilibrium dialysis, atomic absorption spectrometry, fluorescence titration anddetermination of fluorescence lifetime, it can be followed that each fibrinolytic principle(FP)molecule contains one Ca2+ -binding site and one Ca2+. Energy transfer between Tb3+ and theTrp residue in FP was studied through fluorescence spectroscopy. The results show that theCa2+ in a FP molecule can be substitute by a Tb3+. In FP molecule, the excition energy can betransferred from the Trp residue as an energy donor to the Tb3+ as an acceptor, and substitutedinto FP and located near the Trp residue. The distance between Tb3+ and the Trp residue, a0.375 urn, is worked out with the experimental data and Forster theory.

关 键 词：铽 纤溶组分 能量转移 尖吻蝮蛇蛇毒 蛇毒 

分 类 号：R996.3[医药卫生—毒理学]