8%乳化异氟醚后处理对大鼠局灶性脑缺血再灌注损伤的影响  

Effect of 8% emulsified isoflurane postconditioning on focal cerebral ischemia-reperfusion injury in rats

作　　者：朱雯[1] 王志萍[2] 王元琳[1] 

机构地区：[1]徐州医学院江苏省麻醉学重点实验室,221002 [2]徐州医学院附属医院麻醉科

出　　处：《中华麻醉学杂志》2010年第8期996-998,共3页Chinese Journal of Anesthesiology

基　　金：江苏省自然科学基金（BK2009090）

摘　　要：目的 探讨8%乳化异氟醚后处理对大鼠局灶性脑缺血再灌注损伤的影响.方法 健康成年雄性SD大鼠48只,体重260～300 g,随机分为6组（n=8）：假手术组（S组）、缺血再灌注组（I/R组）、低、中、高剂量乳化异氟醚后处理组（L-EI组、M-EI组和H-EI组）和脂肪乳组（LE组）.除S组外,均采用大脑中动脉阻闭2 h,再灌注24 h的方法建立局灶性脑缺血再灌注模型.L-EI组、M-EI组和H-EI组分别于再灌注即刻腹腔注射8%乳化异氟醚3.5、7.0、10.5 ml/kg,LE组给予30%脂肪乳10.5 ml/kg,S组与I/R组不给药.再灌注24 h时行神经功能缺陷评分（NDS）,取脑组织测定脑梗死体积.结果 与S组比较,其余各组NDS评分升高,脑梗死体积增大（P＜0.01）;与I/R组比较,M-EI组和H-EI组NDS评分降低,脑梗死体积减小（P＜0.01）,L-EI组和LE组上述指标差异无统计学意义（P＞0.05）;与M-EI组比较,H-EI组NDS评分降低,脑梗死体积减小（P＜0.05）.结论 8%乳化异氟醚后处理可减轻大鼠局灶性脑缺血再灌注损伤,且与剂量有关.Objective To investigate the effect of postconditioning with 8 % emulsified isoflurane （EI） on focal cerebral ischemia-reperfusion （I/R） injury in rats. Methods Forty-eight male SD rats weighing 260-300 g were randomly divided into 6 groups （ n = 8 each）： group Ⅰ sham operation （group S）; group Ⅱ I/R; group Ⅲ,Ⅳ, Ⅴ 3 different doses of EI （group L-EI, M-EI, H-.EI） and group Ⅵ lipid emulsion （group LE）. Right middle cerebral artery occlusion （MCAO） was induced by inserting a nylon thread 0.24 mm in diameter into right internal carotid artery. The thread was threaded cranially until resistance was met. MCAO was maintained for 2 h followed by 24 h reperfusion in group Ⅱ-Ⅵ. In group Ⅲ-Ⅴ EI 3.5, 7.0 and 10.5 ml/kg were injected intraperitoneally （IP） immediately before reperfusion respectively. In group LE 30% lipid emulsion 10.5 ml/kg was given instead of EI. The neurologic deficit was assessed and scored （0 = normal, 4 = unable to move and unconscious）. The animals were then killed and infarct size was measured. Results IP 8% emulsified isoflurane 7.0 and 10.5 ml/kg injected before reperfusion significantly reduced neurologic deficit scores and infarct size in group M-EI and H-EI as compared with group I/R. Conclusion Postconditioning with 8% emulsified isoflurane can protect the brain against focal cerebral I/R injury.

关 键 词：异氟醚 脂肪乳剂 静脉注射用 再灌注损伤 脑 缺血后处理 

分 类 号：R965[医药卫生—药理学]