光谱、波谱法研究铜锌超氧歧化酶与氯化钴(Ⅱ)、组氨酸钴(Ⅱ)相互作用  

Study on the Interaction of Cu 2Zn 2SOD with CoCl 2,Co(His) n by Spectral and Magnetic Resonance Analysis

作　　者：胡皆汉[1,2,3] 郑学仿[1,2,3] 许永廷[1,2,3] 程国宝 赵永魁[1,2,3] 

机构地区：[1]中国科学院大连化学物理研究所 [2]大连大学化学化工系 [3]辽宁师范大学实验中心

出　　处：《光谱学与光谱分析》1999年第3期304-309,共6页Spectroscopy and Spectral Analysis

基　　金：辽宁省科委自然科学基金

摘　　要：我们首次发现的铜锌超氧歧化酶（Ｃｕ２Ｚｎ２ＳＯＤ）与氨基酸等发生直接相互作用的现象，是一种前人未研究过的重要的生化新现象［１］。在此新发现的基础上，本文用ＩＣＰ，ＶＩＳ，ＮＭＲ和酶活性测定等方法，又从不同角度拓展研究了Ｃｕ２Ｚｎ２ＳＯＤ酶与两类不同化合物，即无机氯化钴（ＣｏＣｌ２）、有机组氨酸钴（Ｃｏ（Ⅱ）（Ｈｉｓ）ｎ）的直接相互作用，发现酶活性中心金属离子同样与外加的这两类不同化合物发生相互作用，相应地影响了酶的催化活性。还发现Ｃｏ（Ⅱ）（Ｈｉｓ）ｎ比ＣｏＣｌ２与酶相互作用更强、更快，Ｃｏ（Ⅱ）（Ｈｉｓ）ｎ中的Ｃｏ（Ⅱ）更易进入酶中，更影响了酶的催化活性。Direct interaction of Cu 2Zn 2SOD with inorganic metal compound(CoCl 2) and organic metal compound[Co(Ⅱ)(His) n]was studied by ICP,VIS,NMR and measurement of enzyme activity.It has been found that in aqueous solution,there exists a direct interaction of the metal ions of the active center in the metalloenzyme(Cu 2Zn 2SOD)with external added CoCl 2,Co(His) n.As a result,part of the metal ions in metalloenzyme were replaced and the corresponding metalloenzyme derivatives were produced and the catalytic activity of enzyme were affected.Moreover.The interaction of Co(Ⅱ)(His) n with Cu 2Zn 2SOD is stronger and quicker,than that of CoCl 2 with this enzyme,as well as the Co(Ⅱ) from Co(Ⅱ)(His) n is easier to enter the active center of enzyme.

关 键 词：铜锌超氧歧化酶 氯化钴 SOD 组氨酸钴 相互作用 

分 类 号：O614.812[理学—无机化学] Q554.902[理学—化学]