天门冬属部分物种种间核糖体基因的ITS序列比较研究  

Comparison of rDNA Internal Transcribed Spacer Sequences in Asparagus

作　　者：欧立军[1,2] 叶威[1] 曾桂萍[3] 蒋向辉[1,2] 佘朝文[1,2] 许栋[1] 杨加强[1] 

机构地区：[1]怀化学院生命科学系,湖南怀化418008 [2]湘西药用植物与民族植物学湖南省高校重点实验室/民族药用植物资源研究与利用湖南省重点实验室,湖南怀化418008 [3]贵州大学农学院,贵州贵阳550025

出　　处：《中药材》2010年第10期1542-1545,共4页Journal of Chinese Medicinal Materials

基　　金：湖南省高校创新平台开放基金项目(09K106);湖南省科技计划重点项目(2009FJ2008)

摘　　要：目的:利用ITS序列对天门冬属9个物种进行分析,鉴定真假天门冬药材和分析天门冬属系统发育。方法:通过对ITS序列进行扩增、克隆测序及序列比对分析。结果:9物种的ITS序列全长为711～748 bp,G+C含量约为60%;以ITS全序列构建的系统树将9个种分为2个亚群:天门冬、石刁柏、武竹、非洲天门冬和狐尾天冬为1亚群,其他4个种为1个亚群;第1个亚群中,来自非洲的非洲天门冬和狐尾天冬优先聚类,然后与非洲天门冬的变种武竹聚为一类;另1亚群中,文竹与其他三种天门冬的亲缘关系相对较远。结论:ITS可以将不同天门冬属物种区分开来,但某些物种在系统树中的划分地位值得商榷,认为ITS序列最好结合其他多种分析手段才能更加准确的分析属内物种的系统发育。Objective： Using ITS sequence of nine species to identify counterfeiting medicine and analyse phylogenetic of Asparagus.Methods： Analysing ITS sequences by amplification,cloning,sequencing and alignment.Results： The length range of ITS sequence of nine species was from 711 to 748 bp,the percentage of G ＋ C content was about 60%.The phylogenetic tree constructed on the basis of the ITS sequences showed that nine species were divided into two branches： Asparagus cochinchinensis,Asparagus officinalis,Asparagus densiflorus,Asparagus densiflorus cv.Myers and Asparagus densiflorus cv.Sprengeri were a branch and the others were a branch.Asparagus densiflorus and Asparagus densiflorus cv.Myers those were from Africa had priority to clustering and then clustering with Asparagus densiflorus cv.Sprengeri that was a variant of Asparagus densiflorus in the first branch.Asparagus setaceus had relatively distant genetic relationship with the others three materials in another branch.Conclusions： The ITS sequences could distinguish species of Asparagus to test the counterfeit.Division status in phylogenetic tree of some species were debatable and ITS sequence was combined with others analytical tools to analyze the realistic phylogeny.

关 键 词：天门冬属 ITS序列 鉴定 系统发育 

分 类 号：R282.71[医药卫生—中药学]