绞股蓝总皂苷对小鼠白血病L1210细胞抑制作用初探  

Suppressive Effect of Gypenosides on Murine Leukemia L1210 Cell Lines

作　　者：杨靓[1,2] 王攀[2] 成晓霞[2] 张孟颖[2] 肖娅萍[1,2] 

机构地区：[1]西部濒危药材开发国家工程实验室 [2]陕西师范大学生命科学学院

出　　处：《中药材》2010年第10期1588-1592,共5页Journal of Chinese Medicinal Materials

基　　金：丹参;绞股蓝和山茱萸优良品种选育研究"十一五"科技支撑计划(2006BAI06A12-04)

摘　　要：目的:探讨绞股蓝总皂苷(Gypenosides,Gyp)对小鼠白血病L1210细胞生长抑制的作用机制。方法:采用MTT法检测Gyp对体外培养的小鼠L1210细胞的增殖抑制作用,流式细胞仪检测Gyp对L1210细胞线粒体膜电位的影响;DAPI染色检测Gyp对L1210白血病细胞核的变化;单细胞凝胶电泳法检测Gyp对白血病L1210细胞DNA损伤的影响。结果:浓度为100～500μg/mL的Gyp对L1210细胞增殖生长有抑制作用,350μg/mL的Gyp作用于L1210细胞0、12、24 h,线粒体膜电位显著下降;Gyp(350μg/mL)作用4 h时,细胞的活性氧产量最高。Gyp(350μg/mL)作用4、12、24 h后,单细胞凝胶电泳观测到DNA损伤;DAPI染色发现细胞核发生变化,并有时间依赖性。结论:Gyp能抑制L1210细胞增殖,这种抑制作用与活性氧的产生、线粒体膜电位下降和DNA损伤有关。Objective： To investigate the suppressive effect of gypenosides（Gyp） on murine leukemia L1210 cells.Methods： The growth inhabitation of murine leukemia L1210 cell was detected by MTT assay.The production of reactive oxygen species and the change of mitochondrial membrane potential were detected by flow cytometry.The change of nuclear and DNA damage of murine leukemia L1210 cells were detected by DAPI staining and single cell gel electrophoresis.Results： Gyp（100 ～ 500 μg/mL） inhibied the growth of murine leukemia L1210 cells.The concentration of Gyp（350 μg/mL） treated murine leukemia L1210 cells at different time points,the mitochondrial membrane potential decrease obviously.L1210 cells were treated with Gyp（350 μg/mL） for 4 h,the highest production of reactive oxygen species was induced.DNA damage were detected after Gyp（350 μg/mL） treated for 4,12,24 h.The change of nuclear was treated by Gyp（350 μg/mL） with time-dependent.Conclusion： Gypenosides has effects on cell viability,induce reactive oxygen species and decreases mitochondrial membrane potential,and can induce morphological changes and DNA damage.

关 键 词：绞股蓝总皂苷 小鼠白血病L1210细胞 抑制 

分 类 号：R285.5[医药卫生—中药学]