转化生长因子-β1基因509C/T多态性在宁夏煤工尘肺人群中的分布  

The Distribution of TGF-β1 Gene-509 C/T Polymorphism in Coal Worker's Pneumoconiosis Patients in Ningxia Area

作　　者：杨文海[1] 杨瑾[1] 王发选[1] 王亚娜[2] 汪岭[1] 陈莉莉[1] 宋琦如[1] 

机构地区：[1]宁夏医科大学公共卫生学院,宁夏银川750004 [2]宁夏医科大学医学科学技术研究中心,宁夏银川750004

出　　处：《环境与职业医学》2010年第11期670-672,共3页Journal of Environmental and Occupational Medicine

基　　金：宁夏医学院预研项目(编号:YY200752);宁夏自然科学基金项目资助(编号:NZ0996)

摘　　要：[目的]探讨转化生长因子-β1(TGF-β1)基因509C/T多态性在宁夏汉族煤工尘肺(coal worker’s pneumoconiosis,CWP)人群中的分布情况。[方法]从宁夏某煤业集团所属煤矿已确诊的病例中随机抽取110名煤工尘肺病例(煤工尘肺组)、110名煤尘接触者(煤尘接触组)和110名当地不接尘的正常居民(非煤尘接触组)为研究对象,采集静脉血,使用聚合酶链反应-限制片段长度多态性(PCR-RFLP)分析TGF-β1基因509CT位点多态性。[结果]煤工尘肺组、煤尘接触组及非煤尘接触组平均年龄分别为(48.30±13.16)岁、(43.86±4.20)岁、(41.73±5.45)岁。煤工尘肺组和煤尘接触组工龄分别为(17.40±7.84)年和(16.95±6.10)年。工种构成:主掘进工在煤工尘肺组和煤尘接触组中分别占48.2%和49.1%。煤工尘肺组和煤尘接触组及非煤尘接触组中吸烟烟龄分别为(10.48±10.12)年、(11.34±10.48)年、(12.65±11.53)年;接尘工龄、工种构成、吸烟烟龄均无差别,均P>0.05。TGF-β1基因509C/T位点多态性在煤工尘肺组、煤尘接触组及非煤尘接触组中所占比例分别为51.8%、30.9%、25.5%;等位基因频率T在煤工尘肺组、煤尘接触组及非煤尘接触组中所占比例分别为40.90%、23.20%、19.10%;煤工尘肺组与煤尘接触组比较,TGF-β1基因中CT基因型差异有统计学意义(P<0.01)。煤工尘肺组不同期别人群的等位基因频率差异有统计学意义(P<0.01)。[结论]TGF-β1-509C/T位点多态性在宁夏地区煤工尘肺者中的分布可能不同。[Objective] To investigate gene polymorphisms of transforming growth factor-beta1（TGF-β1）-509 C/T in patients with coal worker＇s pneumoconiosis（CWP）.[Methods] Total of 110 patients with documented CWP and 110 workers exposed to dust and 110 local residents without dust exposure as control group were recruited.The TGF-β1-509 C/T genotypes were determined by PCR-restriction fragment length polymorphism of vein blood samples.[Results] The average ages of CWP group,the dust exposure group and the control group were（48.30±13.16）（Mean±SD）,（43.86±4.20）,and（41.73±5.45）years respectively;The average working ages of the CWP group and the coal dust exposure group were（17.40±7.84）years（Mean±SD）and（16.95±6.10）years respectively.48.2% of the CWP group and 49.1% of the dust exposure group were drifters.The durations of smoking varied in the CWP group,the dust exposure group and the control group[（10.48±10.12）years（Mean±SD）,（11.34±10.48）years,（12.65±11.53）years,respectively].The length of dust expsure and job title distribution were found no statistically difference between worker＇s groups.Smoking ages were not difference in all three groups.The percentage of TGF-β1-509 C/T genotype polymorphism were 51.8%、30.9% and 25.5% for the CWP group,the dust exposure group and the control group respectively.The allele frequency of T in the CWP group,the dust exposure group and the control group were 40.9%、23.2%、19.1% respectively.Both TGF-β1-509 C/T genotypes and alleles frequencies were significantly higher in the CWP patients than in the controls（P 0.05）.[Conclusion] TGF-β1-509 C/T polymorphisms distributions were different in workers with CWP and residents without dust exposure.

关 键 词：煤工尘肺 转化生长因子 聚合酶链反应-限制片段长度多态性 多态性 

分 类 号：R13[医药卫生—劳动卫生]