反复力竭游泳应激对大鼠海马结构神经元型一氧化氮合酶表达的影响  

Effects of Repeated Stress of Exhausted Swim on Expression of Neuronal Nitric Oxide Synthase in Rat Hippocampal Formation

作　　者：药宏亮[1] 

机构地区：[1]中北大学生理学实验室,山西太原030051

出　　处：《首都体育学院学报》2010年第6期93-96,共4页Journal of Capital University of Physical Education and Sports

摘　　要：为探讨海马结构(海马和齿状回)神经元型一氧化氮合酶(nNOS)与运动疲劳应激的关系,观察反复力竭游泳所引起的疲劳应激对大鼠海马结构nNOS表达的影响。经4周力竭游泳训练制成大鼠运动疲劳应激模型,用免疫组织化学方法显示海马和齿状回nNOS阳性神经元,用图像处理半定量方法对该神经元的数量、面积和灰度进行测量和分析。结果显示:应激实验组大鼠海马和齿状回nNOS免疫阳性神经元数量、面积和灰度均明显大于对照组(P<0.05,P<0.01,P<0.001)。运动疲劳应激可使大鼠海马结构神经元nNOS上调,该神经元参与运动疲劳应激的形成,其机理可能与海马对情绪记忆和齿状回对应激反应的心理行为调节以及NO的神经毒性有关。Objective：To investigate the relationship between exercise-induced fatigue stress and neuronal nitric oxide synthase （nNOS） in hippocampal formation （hippocampus and dentate gyms）. It was observed that the effects of fatigue stress induced by repeating exhaustion swim on expression of nNOS in the rat hippocampal formation. Method： After long-term （4 weeks） swimming exercise till exhaustion, the rats＇ exercise-induced fatigue stress model was made. At rest, the nNOS positive neurons in hippocampus and dentate gyrus were shown hy using ABC immunohistochemistry integrating with image pattern semiquantitative analysis （neuron number,area and gray degree）. Result： A significant increase of nNOS immunoreactive positive neurons in hippocampus and dentate gyrus in the number, area and gray degree in stress group （P〈0. 01 ,P〈0.05, P〈0. 001）. Conclusion： Exercise-induced fatigue stress made up-regulation of nNOS in the rat hippocampal formation, which was involved in forming of fatigue-induced stress, due to the psychological and behavioral regulation of hippocampus on emotion memory and dentate gyrus on stress as well as NO neurotoxicity.

关 键 词：疲劳 应激 海马结构 神经元型一氧化氮合酶(nNOs) 大鼠 

分 类 号：G804.4[文化科学—运动人体科学]