Development of a Synthetic Peptide ELISA Assay for the Detection of Foot-and-Mouth Disease Virus Nonstructural Protein Antibodies  

作　　者：YANG Ji-fei YANG Su-zhen YANG Yan-yan ZHI Ai-min ZHAO Dong ZHI Yu-bao XING Guang-xu DENG Rui-guang CHAI Shu-jun ZHANG Gai-ping 

机构地区：[1]Henan Provincial Key Laboratory for Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, P.R.China [2]College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, P.R.China

出　　处：《Agricultural Sciences in China》2010年第11期1677-1683,共7页中国农业科学（英文版）

基　　金：supported by the National Natural Science Foundation of China (30730068);the National High-Tech R&D Program of China (863 Program,2007AA100606)

摘　　要：In order to develop an ELISA assay with synthetic peptides for the detection of antibody to the nonstructural proteins of foot-and-mouth disease virus, specific peptides were synthesized by a solid-phase method according to FMDV NSPs B-cell epitopes, and were conjugated to carrier protein BSA. An ELISA system was developed to detect FMDV NSPs antibody with the conjugated proteins as the coating antigen. The optimal coating concentration of the antigen was determined as 2.5 μg mL-1. The comparative study of this assay with UBI NSP ELISA kit and national commercial 3ABC ELISA kit in the detection of 199 serum samples showed that they were very coincident, and the identity rates were 96.48 and 97.48%, respectively. The development of ELISA using the synthetic peptides as coating antigen is specific, reproducible, stable, and easy, and can be used to differentiate FMDV infected pigs from immunized pigs.In order to develop an ELISA assay with synthetic peptides for the detection of antibody to the nonstructural proteins of foot-and-mouth disease virus, specific peptides were synthesized by a solid-phase method according to FMDV NSPs B-cell epitopes, and were conjugated to carrier protein BSA. An ELISA system was developed to detect FMDV NSPs antibody with the conjugated proteins as the coating antigen. The optimal coating concentration of the antigen was determined as 2.5 μg mL-1. The comparative study of this assay with UBI NSP ELISA kit and national commercial 3ABC ELISA kit in the detection of 199 serum samples showed that they were very coincident, and the identity rates were 96.48 and 97.48%, respectively. The development of ELISA using the synthetic peptides as coating antigen is specific, reproducible, stable, and easy, and can be used to differentiate FMDV infected pigs from immunized pigs.

关 键 词：foot and mouth disease virus （FMDV） ELISA synthetic peptide 

分 类 号：S855.3[农业科学—临床兽医学] S858.285.3[农业科学—兽医学]