幽门螺杆菌尿素酶基因的克隆及表达  

Cloning and expresssion of the ureB gene from Helicobacter pylori

作　　者：张如华[1] 徐双兵[1] 胡开顺[1] 康铁邦[1] 

机构地区：[1]中山大学肿瘤防治中心实验研究部,广东广州510060

出　　处：《分子诊断与治疗杂志》2010年第6期387-389,共3页Journal of Molecular Diagnostics and Therapy

摘　　要：目的构建幽门螺杆菌尿素酶基因B亚单位(ureB)的原核表达质粒,诱导重组蛋白表达。方法以幽门螺杆菌基因组DNA为模板,PCR扩增尿素酶基因B亚单位,扩增产物经琼脂糖凝胶电泳分离后连接克隆载体并测序,构建重组原核表达载体。结果成功扩增出1710bp的目的基因,测序结果证实与预期一致,该基因在大肠杆菌表达系统中得到表达。结论在大肠杆菌中成功表达幽门螺杆菌尿素酶基因B亚单位蛋白,为幽门螺杆菌疫苗的研发奠定了基础。Objective To construct the recombinant expression vector inserted by ureB gene from Helicobacter pylori and express the UreB protein. Methods The ureB gene was amplified by the PCR reaction from the genomic DNA of Helicobacter pylori. After agarose gel electrophoresis, the ureB gene was cloned into pMD19-T vector and sequenced. In addition, the ureB was inserted into PET-28a（＋） vector to construct recombinant expression vector. Results The ureB gene（1710 bp） was amplified and expressed in E.coli BL21（DE3） successfully. Conclusion The recombinant ant UreB protein was expressed successfully and contributed to the vaccine research of Helicobacterpylori.

关 键 词：幽门螺杆菌 尿素酶 基因克隆 

分 类 号：R378[医药卫生—病原生物学]