扬麦13抗赤霉病品系的分子标记辅助选育  

Breeding the Lines with Resistance to Fusarium Head Blight of Wheat Cultivar Yangmai 13 by Molecular Marker Assisted Selection

作　　者：陆成彬[1,2] 程顺和[2] 吴荣林[2] 胡云花[2] 范金平[2] 王朝顺[2] 张伯桥[2] 

机构地区：[1]扬州大学农学院,江苏扬州225009 [2]江苏里下河地区农业科学研究所,江苏扬州225007

出　　处：《麦类作物学报》2010年第6期1058-1064,共7页Journal of Triticeae Crops

基　　金：国家转基因生物新品种培育重大专项(2008ZX08002-001);国家"973计划"项目(2009CB118300)

摘　　要：通过分子标记辅助选择技术和回交育种方法,以苏麦3号为抗赤霉病基因Fhb1和Fhb2的供体亲本,以弱筋感病品种扬麦13为受体和轮回亲本,对扬麦13进行赤霉病抗性改良。利用抗性基因紧密连锁的SSR标记筛选和田间赤霉病抗性鉴定,获得8个农艺性状似轮回亲本且含有目标基因的品系。通过分子标记对其进行遗传背景分析,获得3个与轮回亲本基本相同的品系。对这3个品系和扬麦13进行赤霉病接种鉴定和主要品质指标检测与比较,最终培育出携带赤霉病抗性基因且保持轮回亲本优良农艺性状及弱筋品质的品系R扬麦13-2、R扬麦13-7和R扬麦13-8,赤霉病病小穗率降低了78.82%～84.58%,产量提高了1 7.24%～26.72%,完全可以替代当前生产上高感赤霉病的扬麦13品种进行推广应用。这表明利用与抗性基因紧密连锁的分子标记辅助育种是一种有效的途径,可以实现小麦赤霉病抗性改良的目标。Molecular marker-assisted method combined with backcrossing was used to improve the disease resistance of Yangmai 13 to Fusarium head blight（FHB） and increase its production and profits. Using the resistant cultivar Sumai 3 as the donor parent of FHB resistance gene Fhb1 and Fhb2,and Yangmai 13 serve as the receptor and the recurrent parent,which was planted largest in the middle and lower reaches of the Yangtze River with weak gluten character and poor resistance to FHB,we successfully characterized eight lines with agronomic traits resemble to Yangmai 13 by using SSR markers liked to resistance genes.Finally,three lines with genetic background consistence with the recurrent parent were identified by genetic molecular markers,which were used for further tests of pathogen inoculation and main quality traits.The three lines developed through this method,were named as R Yangmai 13-2,R Yangmai 13-7 and R Yangmai 13-8,carrying Fhb1,Fhb2,good agronomic traits and soft quality.The disease incidence of them decreased by 78.82%～84.58%and wheat yield improved by 17.24%～26.72%,respectively compared to Yangmai 13,and could replace the susceptible FHB Yangmai 13 for large plantation.This study demonstrates that molecular marker-assisted selection with molecular markers tightly link to the major resistance genes offers opportunities for transferring FHB resistance in wheat breeding.

关 键 词：小麦 赤霉病 标记辅助选择 抗性育种 

分 类 号：S512.1[农业科学—作物学] S330