鸡新城疫病毒东北地区分离株融合蛋白基因的克隆与鉴定  

Clone and Identification of F Gene of Two NDV Isoates from Northeast of China

作　　者：苑纯秀[1] 曹殿军[1] 郭鑫[1] 闵平[1] 孔宪刚[1] 卢景良[1] 

机构地区：[1]中国农科院哈尔滨兽医研究所兽医生物技术国家重点实验室,哈尔滨150001

出　　处：《中国预防兽医学报》1999年第4期287-288,共2页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家85 攀登计划B类项目;国家自然科学基金

摘　　要：以 Ｎ Ｄ Ｖ 东北地区分离株 Ｄ Ｂ３ 、 Ｄ Ｂ５ 的基因组 Ｒ Ｎ Ａ 为模板， 通过 Ｒ Ｔ Ｐ Ｃ Ｒ 技术扩增其 Ｆ 基因的ｃ Ｄ Ｎ Ａ，并将其克隆到质粒ｐ Ｕ Ｃ１９ 中， 转化感受态大肠杆菌 Ｄ Ｈ５α， 经分子量比较、酶切分析、 Ｐ Ｃ Ｒ 等鉴定方法证明， 我们分别获得了含有 Ｎ Ｄ Ｖ Ｄ Ｂ３ 、 Ｄ Ｂ５ 株 Ｆ基因的阳性重组质粒。Genomic RNA was abstracted from purified NDV DB3 and DB5 strains which isolated from Northeast district in China,and used as templates for cDNA synthesis of F gene Then cDNA of F gene were amplified by PCR and cloned into the pUC19 The positive clones of F gene were identified by comparing molecular weight,analysing with Restriction Endonucleases and PCR

关 键 词：新城疫病毒 F基因 克隆 鉴定 

分 类 号：S858.31[农业科学—临床兽医学] S852.65[农业科学—兽医学]