强直性脊柱炎患者外周血单核及T淋巴细胞CD147的表达及意义  

Expressions of CD147 in peripheral monocytes and T lymphocytes of patients with ankylosing spondylitis

作　　者：王敏[1] 黄志祥[1] 潘云峰[1] 张富程[2] 郑奔容[1] 邓伟明[1] 李天旺[1] 古洁若[1] 

机构地区：[1]中山大学附属第三医院风湿科,广州510630 [2]中山大学附属第三医院中心实验室,广州510630

出　　处：《中华医学杂志》2010年第41期2902-2906,共5页National Medical Journal of China

基　　金：广东省科技计划项目（2008B030301039）

摘　　要：目的 观察强直性脊柱炎（AS）患者外周血单核及T淋巴细胞中CD147的表达情况,初步探讨CD147在AS发病及疾病活动中的意义.方法 入组AS患者、类风湿关节炎（RA）患者及健康对照者（HC）各30例,以流式细胞术分析比较各组间外周血单核及T淋巴细胞CD147表达的差异,并经逆转录-聚合酶链反应（RT-PCR）法检测比较其外周血单个核细胞（PBMC）CD147 mRNA表达的差异;进而将AS患者单核及T淋巴细胞CD147表达水平与常用的疾病活动性指标行相关分析.结果 AS、RA及HC组外周血单核细胞CD147的平均荧光强度（MF1）分别为213.5±37.8、228.7±49.7及163.6±44.8,T淋巴细胞CD147的MFI则分别为36.8±10.1、40.2±10.5及28.3±10.6,AS组单核及T淋巴细胞CD147的表达水平均略低于RA患者,但差异均无统计学意义（均P＞0.05）,而AS与RA组单核及T淋巴细胞CD147的表达水平均高于HC组,差异均有统计学意义（均P＜0.05）;AS与RA组PBMC中CD147 mRNA表达水平均高于HC组（P＜0.05）,而AS与RA组间差异无统计学意义（P＞0.05）;AS患者单核及T淋巴细胞CD147 MFI与其ESR及CRP均存在正相关（P＜0.05）.结论 AS患者单核及T淋巴细胞中CD147的表达较正常升高,其水平与患者ESR及CRP存在正相关,提示CD147可能是AS患者疾病发生发展的重要影响因素.Objective To investigate the roles of CD147 in the pathogenesis and development of ankylosing spondylitis（AS）. Methods Flow cytometry was used to detect the expression levels of CD147 in peripheral monocytes and T lymphocytes of 30 AS patients, 30 rheumatoid arthritis（RA）patients and 30 healthy controls（HC）. reverse transcription-polymerase chain reaction（RT-PCR）was used to evaluate the expression levels of CD147 mRNA in peripheral blood mononuclear cells（PBMC）. Then the expression levels of CD147 were compared among the groups. And a correlation analysis was conducted between CD147 levels and disease activity indices of AS patients. Results The mean fluorescence intensities of CD147 in monocytes of AS, RA and HC group were 213.5 ± 37.8, 228.7 ±49.7 and 163.6 ±44.8, and in T lymphocytes 36. 8 ± 10. 1, 40. 2 ± 10. 5 and 28. 3 ± 10. 6 respectively. Both the expression levels of CD147 in monocytes and T lymphocytes of AS patients were slightly lower than those of RA patients. But the differences was not statistically significant（P 〉 0. 05）. Both the CD147 levels in monocytes and T lymphocytes of AS and RA group were significantly higher than those of HC group（P 〈 0. 05）. The expression levels of CD147 mRNA in PBMC of AS and RA group were significantly higher than those of HC group（P 〈 0. 05）while no significant difference was found between AS and RA group（P 〉 0. 05）. Both the expression levels of CD147 in monocytes and T lymphocytes of AS patients were positively correlated with the patients＇ erythrocyte sedimentation rate（ESR）and C-reactive protein（CRP）. Conclusion The expressions of CD147 in peripheral monocytes and T lymphocytes of AS patients are up-regulated and their levels are positively correlated with patients＇ ESR and CRP. It implies that CD147 plays critical roles in the pathogenesis and development of AS.

关 键 词：CD147抗原 单核细胞 T淋巴细胞 脊柱炎 强直性 

分 类 号：R686[医药卫生—骨科学]