羟胺对慢性低氧高二氧化碳大鼠肺动脉高压的影响  

Effect of hydroxylamine on pulmonary arterial hypertension induced by chronic hypoxic hypercapnia in rats

作　　者：刘银花[1] 杨汉文[1] 王小同[1] 金露[1] 祝家群[1] 程英[1] 

机构地区：[1]温州医学院附属第二医院康复、脑科中心,浙江温州325027

出　　处：《中国病理生理杂志》2010年第11期2171-2174,共4页Chinese Journal of Pathophysiology

基　　金：浙江省自然科学基金资助项目(No.Y2080503)

摘　　要：目的:探讨羟胺(HA)对慢性低氧(O2)高二氧化碳(CO2)大鼠肺动脉压的影响。方法:24只清洁级雄性SD大鼠随机分为3组(每组8只):正常对照组(NC)、低O2高CO2+生理盐水组(NS)和低O2高CO2+HA组(HA),NS组和HA组置于常压低O2高CO2舱内(舱内O2浓度维持在9%-11%,CO2浓度为5%-6%),每天8h,每周6d,共4周。入舱前,HA组腹腔注射HA溶液(12.5mg/kg)1mL,NS组腹腔注射1mL生理盐水。4周后,戊巴比妥钠(35mg/kg)腹腔注射麻醉,以右心导管测定大鼠肺动脉平均压(mPAP),分离右心室(RV)和左心室加室间隔(LV+S),计算右心室重量与左心室加室间隔重量的比值[RV/(LV+S)],以光学显微镜观测肺血管结构变化,用分光光度计测定血浆中硫化氢(H2S)的水平,用免疫组织化学方法及RT-PCR技术观察肺小动脉及支气管胱硫醚-γ-裂解酶(CSE)的表达。结果:(1)与NC组相比:NS组和HA组的mPAP、RV/(LV+S)、肺细小动脉管壁面积/管总面积比值(WA/TA)和肺细小动脉中膜厚度(PAMT)明显升高(P<0.05);NS组的血浆中H2S水平、肺小动脉及支气管CSE的含量和CSEmRNA的表达显著降低(P<0.05)。(2)与NS组相比:HA组的mPAP、RV/(LV+S)、肺小动脉管壁面积/管总面积比值(WA/TA)和肺小动脉中膜厚度(PAMT)明显降低(P<0.05);血浆中H2S水平、肺小动脉及支气管CSE的含量和CSE mRNA的表达显著升高(P<0.05)。结论:羟胺通过提高血浆中H2S的水平、肺小动脉及支气管CSE的含量和CSE mRNA的表达和改善肺部血管重构,降低慢性低O2和高CO2所致的大鼠肺动脉高压。AIM： To explore the effects of hydroxylamine on the pulmonary arterial pressure in chronic hypoxic hypercapnic rats. METHODS： Twenty-four male Sprague-Dawley rats were randomly divided into 3 groups （8 rats in each group）： the normal control group （NC）, hypoxic hypercapnia＋normal saline group （NS）, hypoxic hypercapnia＋hydroxylamine group （HA）. The animals in NS and HA groups were kept in the O2 （9%-11%） and CO2 （5%-6%） cabin, 8 h a day and 6 days a week for 4 weeks. Before entering the cabin, the rats in HA group were administered with 1 mL hydroxylamine （12.5 mg/kg） by intraperitoneal injection, while the rats in NS group were given intraperitoneal injection of 1 mL saline solution. The mean pulmonary arterial pressure （mPAP） was measured by external jugular vein cannulation. The heart was removed, and the right ventricle （RV） and the left ventricle plus the septum （LV＋S） were dissected. The ratio of the wet weight of the RV to that of the LV＋S was calculated. The changes of the pulmonary vascular construction were observed under optical microscope. The concentration of H2S in the plasma was measured with a spectrometer. The expression of cystathionine-γ-lyase （CSE） in the pulmonary arterioles and bronchi was measured by immunohistochemistry and RT-PCR. RESULTS： The values of mPAP, RV/（LV＋S）,vessel wall area/total area （WA/TA） and media thickness of pulmonary arterioles （PAMT） in NS group and HA group were significantly higher than those in NC group （P〈0.05）. The level of H2S in the plasma, the content of CSE protein and the expression of CSE mRNA in NC group were significantly lower than those in NS group （P〈0.05）. The values of mPAP, RV/（LV＋S）, WA/TA and PAMT in HA group were significantly lower than those in NS group （P〈0.05）. The level of H2S in the plasma, the content of CSE protein and the expression of CSE mRNA in HA group were significantly higher than those in NS group （P〈0.05）. CONCLUSION： Hydroxy

关 键 词：羟胺 硫化氢 肺动脉高压 大鼠 低氧高碳酸血症 

分 类 号：R543.2[医药卫生—心血管疾病]