七叶皂苷钠体外对大鼠紫外线所致晶状体上皮细胞NF-κB表达的影响  

Intervention effect of aescinate for UV-induced cataract and its impact on NF-κB expression of rat lens epithelial cells

作　　者：李姗姗[1] 鲁建华[1] 张文芳[1] 刘瑜[1] 

机构地区：[1]兰州大学第二医院眼科,中国甘肃省兰州市730030

出　　处：《国际眼科杂志》2010年第12期2250-2252,共3页International Eye Science

摘　　要：目的:探讨在紫外线(ultraviolet,UV)诱导大鼠白内障形成过程中晶状体上皮细胞(lens epithelial cell,LEC)中NF-κB的活化表达及七叶皂苷钠(aescinate)的抑制作用。方法:大鼠晶状体离体培养,分成UV照射A组,七叶皂苷钠处理B,C,D组,用免疫组织化学方法检测紫外线照射后在RPMI1640培养液里培养3,12和24hLECNF-κB的表达水平。结果:UV照射组LECNF-κB表达水平随时间的延长而增强,且与晶状体混浊程度呈正相关;高剂量七叶皂苷钠处理组的NF-κB表达水平较UV照射组、低、中剂量处理组明显减低(P<0.01),中剂量处理组的NF-κB表达水平与UV照射组存在统计学差异(P<0.05),低剂量处理组的NF-κB表达水平与UV照射组无统计学差异(P>0.05)。结论:紫外线可诱导鼠LECNF-κB的活化;七叶皂苷钠可以抑制LECNF-κB的活化表达并存在一定的剂量依赖性。AIM： To investigate the effect of aescinate on the expression of NF-κB and the intervention to the rat cataract by ultraviolet（UV） rays. METHODS： ：The expression of NF-κB was detected by immunohistochemistry in different phase （3, 12 and 24 hours） after the rat lens epithelial cells were cultivated in RPMI 1640 which were exposed to UV radiation. RESULTS： The expression of NF-κB in the UV groups was significantly higher and it was positively correlated with the degree of lens opacity. Compared with the different doses of aescinate groups and the UV radiation treatment group：the expression of NF-κB in the high dose group was lower than that of the mean ,low dose and UV group（P〈 0.01 ）, and there was no significant difference between the low-dose treatment and UV radiation group （P〉 0.05） and there was significant difference between the mean dose treatment and UV radiation groups （P 〈 0.05）. CONCLUSION： UV can induce the activation of NF-κB.Aescinate can inhibit the activation of NF-κB expression and the occurrence of UV-induced cataract in the rat lens epithelial cells and has a dose-dependent effect.

关 键 词：核转录因子-ΚB 晶状体上皮细胞 七叶皂苷钠 紫外线 

分 类 号：R285[医药卫生—中药学]