亚麻EST-SSR信息分析与标记开发  

Flax (Linum sitatissimum L.) EST-SSR Information Analysis and Marker Development

作　　者：龙松华[1] 李翔[2] 邓欣[1] 王玉富[1] 王进[1] 何东锋[2] 陈信波[1,2] 

机构地区：[1]中国农业科学院麻类研究所,长沙410205 [2]湖南农业大学作物基因工程湖南省重点实验室,长沙410128

出　　处：《武汉植物学研究》2010年第5期634-638,共5页Journal of Wuhan Botanical Research

基　　金：中国农业科学院二级杰出人才科研启动经费;农业公益性行业科研专项(nyzx07-018-03)

摘　　要：与基因组SSR相比,以EST为基础的EST-SSR分子标记具有自身的优点。本研究从11240条亚麻(Linum sitatissmum L.)EST序列中检索出877条含有SSR的序列,其出现频率为7.8%。其中以三核苷酸重复出现的频率最高,占总SSR序列的60.1%;其次是二核苷酸重复,占21.9%;四、五和六核苷酸重复占18%。根据这些含SSR的EST序列共设计了73对SSR引物,在8份亚麻材料间通过PCR扩增检测,有63对引物扩增出清晰条带,引物可用率86.3%;有17对引物在8份亚麻材料间显现出多态性,占可扩增引物的26.3%。Compared with SSR markers derived from genomic DNA,the EST-derived SSR markers have remarkable advantages,such as simpler development,greater information and high transferability.In this study,11240 flax ESTs were screened and 877 SSRs were mined out,accounting for 7.8% of the ESTs.Trinucleotide motifs were most abundant（60.1%）,followed by dinucleotides（21.9%）.The AGA/TCT,GA/CT,AAG/TTC,TC/AG,and TA/AT motifs were themain repeat types,accounting for 6.2%,6.2%,5.9%,5.8% and 5.8% of the 877 SSRs respectively.Tetra-,penta-and hexa-nucleotide motifs represented 18% of the SSRs identified.Seventy-three primer pairs were designed from the SSR containing ESTs,and PCR amplifications were performed among eight flax cultivars.Sixty-three primer pairs yielded amplification products,accounting for 86.3% of the total primers.Polymorphisms among the eight cultivars were found in 17 primer pairs,accounting for 26.3% of the primers with amplification products.

关 键 词：亚麻 分子标记 EST SSR 

分 类 号：Q75[生物学—分子生物学]