北京地区儿童生活方式、免疫状态及ORMDL3基因SNPs与哮喘发生的相关性研究  

Association between ORMDL3 Associated SNPs (rs7216389,rs7216558),Life-style,Immune Response and Childhood Asthma in Beijing,China

作　　者：金哲[1] 王金凤[1] 李红[2] 王园园[1] 王强[2] 吴奎武[1] 徐东群[2] 徐东刚[1] 

机构地区：[1]中国人民解放军军事医学科学院基础医学研究所,北京100850 [2]中国疾病预防控制中心环境与健康相关产品安全研究所,北京100021

出　　处：《医学研究杂志》2010年第11期21-24,共4页Journal of Medical Research

基　　金：国家科技支撑计划课题(2006BAII9B05-3)

摘　　要：目的分析ORMDL3基因的SNP rs7216389,rs7216558在中国北京地区儿童中的基因型频率,并调查其与儿童哮喘间的相关性。综合ORMDL3基因相关SNPs、儿童生活方式、IL-4、总IgE和特异性IgE所反映的免疫状态进行与儿童哮喘的相关性分析。方法采用病例-对照研究的方法,通过《国际儿童哮喘及过敏研究(ISSAC)问卷》收集生活方式信息;对聚合酶链式反应(PCR)产物进行序列分析得到SNPs的基因型;酶联免疫吸附法(ELISA)测定IL-4水平;免疫测定法测得儿童总IgE水平;放射性过敏原吸附试验法(RAST)检测特异性IgE水平。使用多元Logistics回归分析ORMDL3基因相关SNPs、生活方式、免疫状态与儿童哮喘的相关性。结果共收集到北京地区220名哮喘患儿及208名对照儿童的血液样本及相关信息。ORMDL3基因SNPs(rs7216389,rs7216558)在病例与对照组中无差异(P>0.05)。哮喘家族史与过敏性哮喘相关(P<0.05),而哮喘家族史、患儿年龄、采暖方式、家庭吸烟习惯、总IgE水平与非过敏性儿童哮喘显著相关(P<0.05)。结论在北京地区,ORMDL3基因SNPs(rs7216389,rs7216558)与儿童哮喘非显著相关。遗传因素与过敏性哮喘显著相关;而在非过敏性哮喘患儿中生活方式及免疫应激状态与哮喘相关,反映了除遗传因素以外,生活方式、免疫状态也对儿童哮喘具有重要影响。Objective To investigate genotype of ORMDL3 associated SNP rs7216389, rs7216558 and analyze the association between these SNPs and childhood asthma in Beijing, China. To perform the association study between ORMDL3 SNPs （rs7216389, rs7216558 ） , life - style, immune response （IL -4, total plasma IgE and specific IgEs） and childhood asthma. Methods We used case -control method to investigate the association between ORMDL3 SNPs （rs7216389, s7216558） , life- style, immune response （IL- 4, total plasma IgE and specific IgEs） and childhood asthma. Information including life -style was gathered from ISSAC questionnaire. SNP genotype was gathered through PCR products sequence analysis. Human I1 -4 was measured through ELISA, and total plasma IgE and specific IgEs were measured through RAST. Chi - squared test and multivariate logistic regression were used to perform the association study. Results 220 asthmatic children and 208 non - allergic controls were recruited from Beijing Children＇s Hospital and Capital Institute of Pediatrics. Blood samples and questionnaires were collected as well. ORMDL3 SNPs （rs7216389, s7216558） were not different between case- control（P 〉 0.05）and they were not associated with childhood asthma. Asthma family history was associated with atopy. Meanwhile asthma family history, life - style （age, the way of heating and smoking habit） , immune response （total plasma IgE） were strongly associated with non - atopy childhood asthma （P 〈 0.05 ）. Conclusion ORMDL3 SNPs （ rs7216389, s7216558） was not associated with childhood asthma in Beijing, China. But the combined association study results showed that genetic factor （asthma family his- tory） was associated with atopy. And genetic factor （asthma family history） , life -style and immune response （total plasma IgE） were significantly associated with non -atopy childhood asthma.

关 键 词：儿童哮喘 病例对照研究 ORMDL3基因 SNPS 相关性分析 

分 类 号：R725.6[医药卫生—儿科]