SPR在检测肺癌血清CEA中的应用  

Role of Surface Plasmon Resonance Assay on Detection of Serum Tumor Marker CEA in Lung Cancer

作　　者：胡卫东[1] 曾昭睿[2] 胡名柏[1] 曾浩然[1] 雷云[2] 徐闻博[1] 

机构地区：[1]武汉大学中南医院肿瘤科,肿瘤生物学行为湖北省重点实验室,武汉430070 [2]武汉大学化学与分子科学学院

出　　处：《肿瘤防治研究》2010年第11期1303-1305,共3页Cancer Research on Prevention and Treatment

基　　金：湖北省科技攻关资助项目(2007AA30B42-2);肿瘤生物学行为湖北省重点实验室科研启动基金资助项目

摘　　要：目的应用表面等离子共振技术(SPR)建立新的血清癌胚抗原(CEA)浓度测定方法,并与化学发光法(ECLIA)比较检测此法对肺癌检出率的能效。方法建立SPR反应工作体系,检测SPR体系精密度,利用CEA抗原标准品获得标准曲线,分别以SPR和ECLIA方法检测肺癌、非肺癌肿瘤、健康对照各40例血清标本中CEA浓度,比较两种方法诊断肺癌的灵敏度和特异度等指标。结果 SPR批间变异度、批内变异度分别为6.7%、7.7%,肺癌组CEA值[SPR法(33.12±28.66)μg/L,ECLIA法(41.10±30.67)μg/L]明显高于对照组[SPR法(1.12±0.87)μg/L,ECLIA法(1.52±1.46)μg/L],差异有统计学意义(P<0.01)。两种方法检测肺癌阳性率差异无统计学意义(P>0.05),肺癌和非肺癌组阳性率差异无统计学意义(P>0.05)。两种方法在诊断肺癌时灵敏度、特异度、准确率、阳性预测值、阴性预测值等相似。检测120例样品CEA浓度SPA平均工作时间(3.0±0.6)min,ECLIA工作时间为(29.1±6.8)min,两者差异有统计学意义(P<0.01)。结论 SPR技术检测肿瘤标志物CEA准确、快捷、可靠、成本低,具有良好的应用前景。Objective To develop a new method：surface plasmon resonance（SPR）technique for the detection of serum carcinoembryonic antigen（CEA） and compare the effects of SPR and electrochemiluminescense immunoassay（ECLIA） on lung cancer diagnosis.Methods The reaction system of SPR was established and the degree of precision of SPR assay was assessed.The serum CEA levels were detected with both SPR assay and ECLIA in 120samples from patients with lung cancer（n= 40）,non-lung cancer（n=40）,and the healthy controls（n=40）.The data were analyzed with statistical software SPSS.Results Variability intergroup and intragroup of SPR was 6.7%and 7.7%,respectively.Mean serum CEA level in lung cancer group（SPR 33.12±28.66μg/L,ECLIA 41.10± 30.67μg/L） was significantly higher than that in control group（SPR 1.12±0.87μg/L,ECLIA 1.52 ±1.46μg/L）,（P〈0.01）,but there was no difference of the positive rate of lung cancer diagnosis between SPR and ECLIA（P〈0.05）.The positive rate of lung cancer diagnosis was similar to that of non-lung cancer diagnosis,either by SPR or ECLIA（P〈0.05）.The sensitivities,specificities,accuracies,positive predictive rates and negative predictive rates between SPA and ELCIA were also similar（62.5%vs.57.5%,70.0%vs.72.5%,67.5%vs.67.5%,51.0%vs.51.1%,84.2%vs.82.4%,P〈0.05）.The mean work time for detection serum CEA with SPA was significantly shorter than that with ECLIA（3.0±0.6min vs.29.1±6.8min,P〈0.01）.Conclusion SPR is a good assay for detection of the serum CEA leve1of lung cancer which has the advantages of sensitivity,reliability,fastness and low cost.This method may further be developed for clinical application.

关 键 词：SPR CEA ECLIA 肺癌 诊断 

分 类 号：R734.2[医药卫生—肿瘤]