母体血浆中白细胞介素11 DNA甲基化和蛋白水平的检测及其临床意义  

Measurement of Interleukin 11 Methylated DNA and Protein Level in Maternal Plasma and Its Clinical Significance

作　　者：马建鸿[1] 张元珍[1] 祝成亮[2] 周春[1] 王燕[1] 陈红[1] 李家福[1] 

机构地区：[1]武汉大学中南医院妇产科,湖北武汉430071 [2]武汉大学人民医院检验科,湖北武汉430060

出　　处：《武汉大学学报（医学版）》2010年第6期792-795,共4页Medical Journal of Wuhan University

基　　金：湖北省科技攻关项目(编号:2007AA301B37-2);武汉市科技攻关项目(编号:200760423158)

摘　　要：目的:检测孕妇血浆中甲基化白细胞介素11DNA(M-IL-11)和非甲基化IL-11DNA(U-IL-11)的表达水平及IL-11蛋白的含量,探讨其在临床中的应用价值。方法:选择早、中、晚期妊娠孕妇各60例,以非妊娠期妇女60例为阴性对照组。提取血浆中的游离DNA,用甲基化特异性PCR(MSP)方法检测各组血浆标本中M-IL-11和U-IL-11的表达水平,并采用ELISA的方法检测IL-11的水平,对妊娠组和对照组样本均数进行统计学分析。结果:非妊娠妇女血浆中未检测到U-IL-11,妊娠早期、中期、晚期及非妊娠妇女M-IL-11DNA浓度无显著性差异(P>0.05),而中期、晚期妊娠妇女U-IL-11的水平明显高于早期妊娠妇女(P<0.05)。IL-11水平在孕妇组显著升高,且随着孕周时间延长,其含量不断升高。结论:M-IL-11不是妊娠特异性标志物,U-IL-11可作为妊娠特异性标志物,IL-11的检测有助于扩大非创伤性产前诊断的临床应用范围。Objective：To measure the levels of methylated interleukin11DNA（M-IL-11）and unmethylated IL-11 DNA（U-IL-11）in maternal plasma as well as IL-11 protein,and to explore their diagnostic value in clinical application.Methods：Pregnant women including pregnancy of the first trimester（60cases）,the second trimester（60cases）and the third trimester（60cases）were selected randomly as study group,and sixty normal non-pregnant women were selected as control group.DNA from plasma samples was extracted.The methylation specific PCR（MSP）method was used to detect the expression of M-IL-11DNA and U-IL-11 DNA,and IL-11 levels was measured by ELISA method.Results：U-IL-11 DNA was not deteced in non-pregnant women,there were no statistical significances（P0.05）among the three trimesters,while U-IL-11 in subjects of the second and the third trimester was much higher than that of the first trimester.The levels of IL-11 was increased in pregnant women,and elevated with pregnant time.Conclusion：U-IL-11 but not M-IL-11 is a specific marker of pregnacy,and measurement of IL-11 helps explore the clinic application of noninvasive prenatal diagnosis.

关 键 词：甲基化IL-11 DNA 非甲基化IL-11 DNA 甲基化特异性PCR 

分 类 号：R715.5[医药卫生—妇产科学]