猕猴肠侵袭型大肠杆菌的分离鉴定及其毒力因子基因的检测  

Isolation and identification of enteroinvasive Escherichia coli in Macaca mulatta and detection of its virulence factor gene

作　　者：钟妮娜[1,2] 徐顶[1] 胡刚 彭卫平[1] 陈志荣 杨军[1] 

机构地区：[1]四川农业大学动物医学院,四川雅安625014 [2]动物疫病与人类健康四川省重点实验室,四川雅安625014 [3]成都猕猴繁育研究基地,四川成都610081 [4]黑水县疫病预防控制中心,四川黑水623500

出　　处：《中国兽医科学》2010年第11期1106-1109,共4页Chinese Veterinary Science

基　　金：教育部"长江学者和创新团队发展计划"创新团队项目(IRT0848)

摘　　要：2010年5月从四川省某猕猴养殖场急性腹泻猕猴肠道内分离出4株致病性大肠杆菌,并对其进行了16SrDNA扩增、生化反应、血清型鉴定、药敏试验以及毒力因子基因的分子生物学检测。结果显示,该菌株血清型为O28;药敏试验证实该菌对多种抗生素具有抗性,并且呈现多重耐药性;攻毒试验证实该菌能够导致小白鼠大量死亡;多重PCR成功检测出侵袭性大肠杆菌的Einv毒力基因。确定该病原菌为肠侵袭型大肠杆菌,而且毒力很强。Four Escherichia coli strains were isolated from Macaca mulatta with severe diarrhea on a farm in Sichuan Province in May,2010.Identification of the isolated strains was conducted by detecting 16S rDNA,biochemical reaction,serological test,drug sensitivity test and detecting toxicity gene by molecular biological method.The serotype of these bacteria was O 28 identified by serological test.In drug sensitivity test,the strains showed multidrug resistance,and they could cause mouse to death.Einv gene was detected successfully in the strains by multi-PCR.These results showed that these clinical isolates were virulent enteroinvasive E.coli.

关 键 词：肠侵袭型大肠杆菌 猕猴 毒力因子基因 分离鉴定 

分 类 号：S852.612[农业科学—基础兽医学]