广州市2009年Ⅰ型登革病毒E基因序列分析  

Analysis on envelope gene of type Ⅰ dengue virus isolated from Guangzhou area in 2009

出　　处：《中华传染病杂志》2010年第11期641-644,共4页Chinese Journal of Infectious Diseases

摘　　要：目的 测定广州市2009年Ⅰ型登革病毒的E基因序列并进行分析,探讨其来源及基因型.方法 收集广州市2009年登革热患者急性期血清19份,用C6/36细胞培养分离登革病毒,RT-PCR法扩增全长E基因,测定序列并绘制系统发生树,结合流行病学资料进行信息学分析.结果 19份标本中分离到4株Ⅰ型登革病毒株,测序获得其E基因序列.分析发现4株病毒来自两个不同的亚型,09/GZ/9104和09/GZ/9236两株Ⅰ型登革病毒核苷酸序列相同,属于美洲/非洲型;09/GZ/11534和09/GZ/11562两株Ⅰ型登革病毒序列同源性较高,属于亚洲型.结论 广州市2009年Ⅰ型登革病毒属输入性,分属两个基因型,推测可能来源于不同的传染源.Objective To sequence and analyze the envelope （E） gene of type Ⅰ dengue virus isolated from Guangzhou in 2009 for tracing the infection source. Methods The serum samples were collected from patients diagnosed with dengue fever in Guangzhou area during 2009. Dengue virus was isolated and cultured in C6/36 cells.The whole length of E gene was amplified by reverse transcriptase-polymerase chain reaction （RT-PCR） and then sequenced. The phylogenetic tree was drawn by neighbor-joining method. The bioinformatics analysis was performed by combining the phylogenetic information and the epidemiology data. Results Four strains of type Ⅰ dengue virus were isolated from 19 samples. E gene of these strains was amplified and sequenced. The phylogenetic analysis showed that 09/GZ/9104 strain and 09/GZ/9236 strain had identical nucleotide sequence and fell within the American/African group, 09/GZ/11534 stain and 09/GZ/11562 strain had similar sequence homology and fell within the Asian group. Conclusion The typeⅠdengue viruses in Guangzhou area in 2009 are imported, which belong to two genotypes and may come from two independent origins respectively.

关 键 词：2009年 基因序列分析 登革病毒 广州市 Ⅰ型 C6/36细胞 RT-PCR法 E基因序列 

分 类 号：R686[医药卫生—骨科学]