Preparation of a gene-engineering mutant of bacteriorhodopsin BR-D96V and corresponding poly(vinyl alcohol)-based functional composite films  

作　　者：ZHAO YingChun MING Ming HONG Jie MA DeWang WU Jia LI QingGuo HUANG WeiDa DING JianDong 

机构地区：[1]Key Laboratory of Molecular Engineering of Polymers of Ministry of Education, Department of Macromolecular Science, Fudan University, Shanghai 200433, China [2]Department of Biochemistry, School of Life Sciences, Fudan University, Shanghai 200433, China [3]Department of Physiology and Biophysics, School of Life Sciences, Fudan University, Shanghai 200433, China

出　　处：《Chinese Science Bulletin》2010年第33期3825-3830,共6页

基　　金：supported by the National Basic Research Program of China(2009CB930000);National Natural Science Foundation of China(50533010)

摘　　要：Bacteriorhodopsin(BR)exhibits,as a membrane protein in Halobacterium salinarum,unique photoresponsive behaviors,and shows promise as a functional information material.A new mutant of BR with the 96th aspartic acid replaced by valine(BR-D96V)was obtained and then a composite film of BR-D96V in a synthetic polymer matrix was prepared in this research.The mutant BR-D96V was expressed in a bacterio-opsin deficient halobacterial strain(L33)by gene engineering.Although valine is very hydrophobic,this point mutant keeps the basic biological activities,namely,photoelectric and photochromic responses.Nevertheless,the lifetime of M intermediate in the BR mutant is nearly two orders of magnitude longer than that of wild-type BR in neutral aqueous solution,which benefits its potential application as an information material.The M lifetime is further significantly prolonged after embedding BR-D96V into poly(vinyl alcohol)(PVA).It was also found that BR-D96V is very sensitive to water content in comparison with wild-type BR and another BR mutant.Bacteriorhodopsin （BR） exhibits, as a membrane protein in Halobacterium salinarum, unique photoresponsive behaviors, and shows promise as a functional information material. A new mutant of BR with the 96th aspartic acid replaced by valine （BR-D96V） was obtained and then a composite film of BR-D96V in a synthetic polymer matrix was prepared in this research. The mutant BR-D96V was expressed in a bacterio-opsin deficient halobacterial strain （L33） by gene engineering. Although valine is very hydrophobic, this point mutant keeps the basic biological activities, namely, photoelectric and photochromic responses. Nevertheless, the lifetime of M intermediate in the BR mutant is nearly two orders of magnitude longer than that of wild-type BR in neutral aqueous solution, which benefits its potential application as an information material. The M lifetime is further signifi- cantly prolonged after embedding BR-D96V into poly（vinyl alcohol） （PVA）. It was also found that BR-D96V is very sensitive to water content in comparison with wild-type BR and another BR mutant.

关 键 词：细菌视紫红质 聚乙烯醇 基因工程 突变体 复合膜 BR 制备 聚合物基体 

分 类 号：S511[农业科学—作物学] Q63[生物学—生物物理学]