Acute and chronic un-ionized ammonia toxicity to ‘all-fish’ growth hormone transgenic common carp(Cyprinus carpio L.)  

作　　者：GUAN Bo HU Wei ZHANG TangLin DUAN Ming LI DeLiang WANG YaPing ZHU ZuoYan 

机构地区：[1]State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China [2]Graduate School of Chinese Academy of Sciences, Beijing 100049, China

出　　处：《Chinese Science Bulletin》2010年第35期4032-4036,共5页

基　　金：supported by the National Basic Research Program of China (2007CB109205,2009CB118804);the National Natural Science Foundation of China (30623001)

摘　　要：Ammonia is toxic to fish in natural and artificial waters.We evaluated the acute(96 h) and chronic(21 d) toxicity of un-ionized ammonia to GH transgenic common carp(Cyprinus carpio L.) and non-transgenic common carp using a static-renewal bioassay.The 24,48,72 and 96 h median lethal concentrations(LC50) of un-ionized ammonia were slightly lower in transgenic carp(2.64,2.44,2.28 and 2.16 mg N/L,respectively) than in non-transgenic carp(2.70,2.64,2.52 and 2.33 mg N/L,respectively).Similarly,the median lethal time(LT50) was significantly shorter for transgenic carp(1.41,7.91 and 117.42 h) than for non-transgenic common carp(2.53,14.06 and 150.44 h) following exposure to 3.86,3.29,or 2.09 mg N/L,respectively.Moreover,the mortality of transgenic carp was significantly higher than that of non-transgenic carp at all un-ionized ammonia concentrations((0.91 ± 0.12),(0.48 ± 0.06) and(0.12 ± 0.01) mg N/L) during the 21 d chronic toxicity test.Our results suggest that GH transgenic carp are less tolerant of unionized ammonia than non-transgenic carp.Our data are useful for evaluating potential environmental risk,optimizing stocking density in intensive aquaculture and establishing water quality criteria for ammonia in aquaculture.Ammonia is toxic to fish in natural and artificial waters. We evaluated the acute （96 h） and chronic （21 d） toxicity of un-ionized ammonia to GH transgenic common carp （Cyprinus carpio L.） and non-transgenic common carp using a static-renewal bioassay. The 24, 48, 72 and 96 h median lethal concentrations （LCs0） of un-ionized ammonia were slightly lower in transgenic carp （2.64, 2.44, 2.28 and 2.16 mg N/L, respectively） than in non-transgenic carp （2.70, 2.64, 2.52 and 2.33 mg N/L, respectively）. Similarly, the median lethal time （LTso） was significantly shorter for transgenic carp （1.41, 7.91 and 117.42 h） than for non-transgenic common carp （2.53, 14.06 and 150.44 h） following exposure to 3.86, 3.29, or 2.09 mg N/L, respectively. Moreover, the mortality of transgenic carp was significantly higher than that of non-transgenic carp at all un-ionized ammonia concentrations （（0.91± 0.12）, （0.48 ± 0.06） and （0.12 ±0.01） mg N/L） during the 21 d chronic toxicity test. Our results suggest that GH transgenic carp are less tolerant of un-ionized ammonia than non-transgenic carp. Our data are useful for evaluating potential environmental risk, optimizing stocking density in intensive aquaculture and establishing water quality criteria for ammonia in aquaculture.

关 键 词：慢性毒性试验 生长激素基因 非离子氨 鲤鱼 急性 半致死浓度 转基因鲤 非转基因 

分 类 号：S828.2[农业科学—畜牧学] TQ450.26[农业科学—畜牧兽医]