CD25及FOXP3在支气管哮喘小鼠中的表达以及地塞米松的干预作用  

The Expression of CD25 and FOXP3 in Mouse Asthma and the Effect of Dexamethasone

作　　者：马祥[1] 梁宗安[2] 毛辉[2] 刘雅[1] 王茂筠[2] 

机构地区：[1]成都市第二人民医院老年干部科,成都610031 [2]四川大学华西医院呼吸内科,成都610041

出　　处：《四川大学学报（医学版）》2010年第6期955-959,共5页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30871119)资助

摘　　要：目的分析哮喘小鼠模型肺组织及骨髓中CD25及FOXP3的表达,以及地塞米松的干预作用。方法 BALB/c小鼠随机分为正常对照、哮喘及地塞米松干预组,通过HE染色观察肺组织的病理变化,应用Western blot、RT-PCR方法检测肺组织FOXP3及CD25的表达,RT-PCR方法检测哮喘及地塞米松干预组肺及骨髓FOXP3 mRNA表达。结果哮喘及地塞米松干预组肺组织FOXP3表达强于正常对照组(P<0.05),地塞米松干预促进FOXP3表达(P<0.05);哮喘及地塞米松干预组肺组织CD25表达强于正常对照组(P<0.05),而两组之间无明显差异(P>0.05);哮喘及地塞米松干预组肺组织及骨髓细胞均有FOXP3 mRNA表达,且地塞米松组强于哮喘组(P<0.05)。结论哮喘小鼠肺内CD25及FOXP3表达增强,地塞米松会促进其表达;哮喘小鼠骨髓有FOXP3表达,地塞米松促进其表达。Objective To detect the expression of CD25 and FOXP3 in mouse asthma model, and the effect of dexamethasone （DXM） on it. Methods BALB/e mice were randomly divided into three groups which were of normal control group, asthmatic group and DXM treatment group. The pathological changes of lungs were detected by HE staining. The expressions of CD25 and FOXP3 from lungs were evaluated by Western blot and RT-PCR. The expression of FOXP3 from bone marrow of asthmatic group and DXM treatment group were detected by RT-PCR. Results The expression of FOXP3 in asthmatic group and DXM treatment group was significantly higher than that of normal control group （P〈0. 05）, DXM could promote the expression of FOXP3 （P〈0.05）. The expression level of CD25 in asthmatic group and DXM treatment group was higher than that of normal control group （P〈0. 05）, but no significant difference was observed between asthmatic and DXM treatment groups （P〉0.05）. Expressed FOXP3 mRNA in bone marrow were detected in both asthmatic and DXM treatment groups, but higher expression level was observed in DXM treatment group. Conclusion The expression of CD25 and FOXP3 increased in mouse asthma model, DXM could promote the expression. Cells in bone marrow could express FOXP3, DXM maybe promote the expression of FOXP3.

关 键 词：支气管哮喘 CD25 FOXP3 

分 类 号：R562.25[医药卫生—呼吸系统]