盐胁迫下构树DREB转录因子基因表达的实时荧光定量PCR分析  

DREB Gene Expression in Leaves of Broussonetia papyrifera Seedlings under Salt Stress Detected by Real-Time Fluorescent Quantitative PCR

作　　者：杨帆[1,2] 丁菲[2] 杜天真[2] 

机构地区：[1]湖州师范学院生命科学学院,湖州313000 [2]江西农业大学园林与艺术学院,南昌330045

出　　处：《林业科学》2010年第4期146-150,共5页Scientia Silvae Sinicae

基　　金：国家林业局重点研究项目"构树种质资源及多目标利用营林技术研究"(2006-24);教育部高等学校博士点专项基金"构树特殊抗逆性的生理及分子机理研究"(20060410002)

摘　　要：转录因子是指那些专一性地结合于DNA特定序列上、能激活或抑制其他基因转录的蛋白质（王少峡等,2004）。DREB（dehydration responsive element binding protein）转录因子是一种特异性的转录因子,当植物在干旱、低温及盐等逆境胁迫下,Real-time fluorescent quantitative PCR was used to datect DREB genes＇ expression levels in leaves of Broussonetia papyrifera seedlings at 0,6,12 and 24 h after treatments with 100,200 and 300 mmol·L -1 NaCl. The results showed that BpDREB1 and BpDREB2 genes were induced by 100 and 200 mmol·L -1 NaCl to increase their expression levels. But the levels decreased at later stage by the salt stress. Treatment with 300 mmol·L -1 NaCl caused reduced expression levels of the two genes. The experiment verified that the transcriptional expression of DREB genes in B. papyrifera was able to be induced by salt stress,which might be involved in signalling pathways in response to the salt stress. But the genes were prohibited by higher salt concentration or longer duration of NaCl stress.

关 键 词：实时荧光定量PCR 构树 DREB转录因子 CT值 盐胁迫 

分 类 号：S718.46[农业科学—林学] S718.43