大剂量甲基强的松龙对大鼠急性脊髓损伤后胶质纤维酸性蛋白的影响  

The Effect of Highdose of Methylprednisolone on Glial Fibrillary AcidicProtein after Acute Spinal Cord Injury in Rats

作　　者：焦鹰[1] 王星铎[1] 王泰增[1] 原银栋[1] 朱悦[1] 

机构地区：[1]中国医科大学第一临床学院骨科

出　　处：《中国医科大学学报》1999年第3期181-183,共3页Journal of China Medical University

摘　　要：目的：探讨大剂量甲基强的松龙（ＭＰＳ）治疗大鼠急性脊髓损伤（ＡＳＣＩ）后，胶质纤维酸性蛋白（ＧＦＡＰ）的变化。方法：４４只成年雄性Ｗｉｓｔａｒ大鼠，随机分成对照、损伤和治疗３组。采用Ａｌｅｎ打击法损伤脊髓。损伤组与治疗组于损伤后１５ｍｉｎ内尾静脉内推注ＭＰＳ３０ｍｇ／ｋｇ，以后２３ｈ内静滴ＭＰＳ５．４ｍｇ／ｋｇ·ｈ－１。各组动物分别于损伤后１、４、８ｄ处死，行ＧＦＡＰ单克隆抗体免疫细胞化学染色。结果：对照组大鼠应用ＭＰＳ后４ｄ，ＧＦＡＰ减少到８０％，８ｄ后减少到６３％。ＡＳＣＩ后，各组ＧＦＡＰ增加，治疗组与损伤组ＧＦＡＰ之比损伤后第１ｄ为８７．２％，第４ｄ为８８．６％，第８ｄ为９５．７％。结论：大剂量ＭＰＳ抑制未损伤大鼠ＧＦＡＰ的合成。对ＡＳＣＩ后，ＧＦＡＰ含量的增加也有一定的抑制作用。Objective: Our aim was to study the effect of highdose of methylprednisolone on glial fibrillary acidic protein after acute spinal cord injury in rats. Methods: Immunocytochemistry and image analysis methods were used to detect the change of the glial fibrillary acidic protein (GFAP) in astrocyte in acute spinal cord injury rats which were early treated by intravenous highdose of methylprednisolone. 44 male Wistar rats were randomly assigned to control,injury and experiment groups. Acute spinal cord injury was caused by Allen method. The rats in the control and experiment groups received a bulos of methyprednisolone(30 mg/kg) within 15 min after injury and then received an infusion of methylprednisolone at 5.4 mg/kg per hour for 23 hours. Rats were sacrificed at 1,4 and 8 days postoperatively.GFAP monoclone antibody was used to localized astrocyte by SP method of immunocytochemistry. Results: The results revealed that the area of control rats was reduced to 80% at 4 days and to 63% at 8 days after administration and that the ratio of the area of rats in the experiment group to that in the injury group was 87.5% at lst day, 88.6% at 4 days and 95.7% at 8 days postoperatively. Conclusion: The result indicated that highdose of methylprednisolone could depress the production of GFAP in intact rats and could exert some influence on the increase of GFAP after acute spinal cord injury.

关 键 词：脊髓损伤 甲基强的松龙 胶质纤维 酸性蛋白 GFAP 

分 类 号：R651.2[医药卫生—外科学] R977.1[医药卫生—临床医学]