黄芪对单线态氧清除作用实验研究  

Scavenging Effect of Astragalus Membranaceus on Singlet Oxygen in Isolated Rabbit Hearts

作　　者：徐世安[1,2,3] 徐斌[1,2,3] 康健 郑有仁[1,2,3] 

机构地区：[1]中国医科大学第二临床学院心脏外科 [2]沈阳军区第二○二医院 [3]沈阳军区总医院

出　　处：《中国医科大学学报》1999年第3期184-185,共2页Journal of China Medical University

摘　　要：目的：探讨黄芪对单线态氧的作用。方法：应用兔离体心肌缺血再灌注损伤模型，将心脏随机分成对照组、缺血组和黄芪组（每组５只）。对照组心脏不给予缺血，只给予有氧灌注４０ｍｉｎ；另两组心脏在常温下（３７℃）给予３０ｍｉｎ缺血和１０ｍｉｎ再灌注；黄芪组在Ｔｈｏｍａｓ液中加入黄芪注射液（５ｍｌ／１００ｍｌ）。实验过程中检测心肌组织中的ＭＤＡ以及外部－５′－核苷酸酶的活性。结果：黄芪能有效地保护外部－５′－核苷酸酶的活性，使ＭＤＡ含量明显减少（Ｐ＜０．０５）。结论：在缺血心肌再灌注过程中，黄芪可能具有清除单线态氧的作用。Objective: Our aim was to study the scavenging effect of Astragslus membranaceus on singlet oxygen during ischemia and reperfusion for the isolated rabbit heart. Methods: Applying the experimental models of isolated rabbit myocardial ischemia/reperfusion,we rondomly assigned the isdated rabbit hearts into three groups (n=5/group). The ecto5nucleotidase activity and MDA in myocardial tissue were measured. WT5HZResults: Astragali injection could effectively protect ecto5nucleotidase activity(P<0.05) and largely reduced the amount of MDA (P<0.05). Conclusion: During the course of ischemia and reperfusion,Astagalus membranaceus might have the ability of scavenging singlet oxygen.

关 键 词：黄芪 核苷酸酶 单纯态氧 心肌缺血 再灌注损伤 

分 类 号：R654.2[医药卫生—外科学] R285.5[医药卫生—临床医学]