二磷酸腺苷对肾上皮细胞的细胞骨架的作用  

The Role of Adenosine Diphosphate on the Cytoskeleton of Kidney Epithelial Cells

作　　者：任青[1] 严海东[1] 马健飞[1] 李子龙[1] 吴岚[1] 苏晓林[1] 

机构地区：[1]中国医科大学第一临床学院肾内科

出　　处：《中国医科大学学报》1999年第3期206-207,212,共3页Journal of China Medical University

基　　金：卫生部科研基金

摘　　要：目的：探讨二磷酸腺苷（ＡＤＰ）对细胞的促生长作用。方法：用间接免疫荧光法观察ＡＤＰ对ＢＳＣ－１细胞骨架成分的影响。结果：在用ＡＤＰ前，荧光下非洲绿猴肾上皮细胞（ＢＳＣ－１）的细胞骨架中角质蛋白纤维成分８（Ｃｙｔｏｋｅｒａｔｉｎ８，ＣＫ８）以星形位于细胞核周围，ＡＤＰ作用２ｍｉｎ后，ＣＫ８开始伸长，６ｈ后伸长到最大程度，充满整个细胞，１２ｈ后开始萎缩，２４ｈ后ＣＫ８又以星形位于细胞核的周围。而其它细胞骨架成分未见变化。结论：ＡＤＰ是肾小管上皮细胞最强的一种有丝分裂源。Objective: Our aim was to evaluate the role of adenosine diphosphate (ADP) on the cytoskeleton of kidney epithelial cells. HZMethods: We used indirect immunoflurescence method to observe the influence of ADP on the cytoskeleton of BSC1 cells. Results: In untreated confluent cells, cytokeratin 8 (CK8) was predominantly confined to starlike spot in the area around the nucleus, but the spot began to spread within 2 minutes after addition of ADP. This process completed at the 6th hour after addition of ADP and a welldeveloped filamentous network throughout the cell formed. At the 12th hour, the network began to collapse and the filaments formed a spot again in the perinuclear area. The process completed by the 24th hour. We did not find any change of other cytoskeleton. Conclusion: Exogenous adenosine diphosphate (ADP) was the most potent growth mitogen for nontransformed kidney epithelial cells of BSC1 line.

关 键 词：二磷酸腺苷 细胞骨架 CK8 肾上皮细胞 

分 类 号：R977[医药卫生—药品]