超微脑得健对脑缺血大鼠脑内Rho表达的影响  

Effect of ultramicro Naodejian on Rho expression of cerebral ischemia in rats

作　　者：杜可[1] 易健[1] 刘柏炎[1] 蔡光先[1] 

机构地区：[1]湖南中医药大学,湖南长沙410208

出　　处：《湖南中医药大学学报》2010年第9期50-53,共4页Journal of Hunan University of Chinese Medicine

基　　金：国家自然科学基金资助项目(30873355);教育部重点项目(209087);湖南省科技厅项目(2010SK3015)

摘　　要：目的探讨超微脑得健对脑缺血大鼠脑内Rho的影响,揭示其可能作用机制。方法采用脑中动脉阻塞法复制大鼠局灶性脑缺血模型,将动物随机分为假手术组、模型组、补阳还五汤组、脑得健原方组、超微脑得健全量组,超微脑得健1/3剂量组。采用RT-PCR法检测大鼠脑组织Rho mRNA表达。结果假手术组脑内有一定水平Rho mRNA表达,模型组Rho mRNA表达至7 d达到高峰。给药3d后各治疗组动物脑组织Rho mRNA含量较同时段模型组比较明显降低,差异具有统计学意义(P<0.05)。结论超微脑得健能抑制脑缺血后Rho表达,可能为其促进脑缺血后神经功能恢复作用机制之一。Objective To study the effect of ultramicro Naodejian（UNDJ） on Rho expression of cerebral ischemia in rats to investigate the possible anti-ischemic mechanism.Methods The focal cerebral ischemia rats model was induced by middle cerebral arter occlusion（MCAO）,All rats were randomly divided into 6 groups including shame-operated,model,Buyang Huanwu decoction（BYHWT）,NDJ,UNDJ and 1/3 dose UNDJ groups.The expressions of Rho were measured by RT-PCR.Results The Rho mRNA expressions existed in shame-operation group,and the expression reached to peak at 7 day in model group.The Rho mRNA levels in all treatment groups were lower than that in model group after feeding drugs 3 d（P0.05）.Conclusions UNDJ can inhibit Rho expression after cerebral ischemia,which maybe one of anti-ischemic mechanism to return brain functions.

关 键 词：超微脑得健 脑缺血 脑中动脉阻塞 大鼠 小G蛋白Ras超家族Rho 补阳还五汤 黄芪 地龙 

分 类 号：R285.5[医药卫生—中药学]