散血明目片对兔视网膜静脉阻塞热休克蛋白70表达的影响  

Effect of Sanxue Mingmu tablets on expression of HSP70 in rabbits with retinal vein occlusion

作　　者：彭清华[1] 叶群如[1] 张波涛[1] 姚小磊[1] 谭涵宇[1] 曾志成 韩琦[3] 

机构地区：[1]湖南中医药大学第一附属医院眼科学重点学科,湖南长沙410007 [2]湖南省桂阳县人民医院眼科,湖南桂阳424400 [3]辽宁省沈阳市第一人民医院眼科,辽宁沈阳110041

出　　处：《湖南中医药大学学报》2010年第9期61-63,共3页Journal of Hunan University of Chinese Medicine

基　　金：湖南省科技厅科研基金资助项目(02SSY3096);湖南省中医药科研基金资助项目(2010012);国家中医药管理局中医眼科学重点学科建设项目;湖南省中医五官科学重点学科建设项目

摘　　要：目的观察活血通脉利水明目之散血明目片对兔实验性视网膜静脉阻塞视网膜组织中热休克蛋白70(HSP70)表达的影响。方法将36只家兔(72只眼)随机分为健康空白组、模型组、散血明目片组3组,每组12只(24只眼)。模型组和散血明目片组兔采用激光光凝视网膜中央静脉造模法制造RVO动物模型。健康空白组与模型组每日以生理盐水5 mL/kg灌胃,散血明目片组每日以散血明目片混悬液5 mL/kg灌胃,均灌胃2周,1次/d。采用免疫组化法观察兔视网膜组织中HSP70的表达。结果造模后第3天,散血明目片组及模型组HSP70表达明显升高,与空白组比较差异均具有统计学意义(P<0.01),但散血明目片组与模型组之间差异无统计学意义(P>0.05)。造模后7 d,各组间差异具有统计学意义(P<0.01)。造模后14 d,散血明目片组与模型组及空白组间比较差异均具有统计学意义(P<0.01),但模型组与空白组间比较差异无统计学意义(P>0.05)。结论散血明目片能明显增强实验性视网膜静脉阻塞兔视网膜组织中HSP70的表达。Objective To observe the effect of Sanxue Mingmu tablets（SXMMT） with actions of activating blood,relieving meridians,eliminating dampness and promoting vision on expression of heat shock protein70（HSP70） in rabbits with retinal vein occlusion.Method The 36 rabbits with 72 eyes were divided randomly into 3 groups,namely black group,model group and SXMMT group,12 rabbits with 24 eyes per group.The RVO models in SXMMT and model groups were made by laser photocoagulation.The animals in black and model groups were fed with NS 5 mL/kg and in SXMMT group with the drug 5 mL/kg,feeding 2 weeks and one time a day.The expression of HSP 70 were detected with immunohistochemistry.Results The difference bwtween SXMMT group and black group was significant（P0.01）,but bwtween SXMMT group and model group was not（P0.05） after modeling 3 days.The differences bwtween SXMMT group and other groups were significant（P0.01） after modeling 7 days.And the differences bwtween SXMMT group and other groups were significant（P0.01）,but bwtween SXMMT group and model group was not significant（P0.05） after modeling 14 days.Conclusion SXMMT can strenthen the expression of HSP70 in the model rabbits.

关 键 词：散血明目片 活血通脉利水明目法 视网膜静脉阻塞 热休克蛋白70 兔 

分 类 号：R285.5[医药卫生—中药学]