金银花与山银花HPLC指纹图谱比较研究  

Comparison of HPLC fingerprint profiles of Lonicera Japonica and Lonicera confusa DC.

作　　者：熊艳[1,2] 朱晶晶[1] 王智民[1] 高慧敏[1] 彭新君[2] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]湖南中医药大学,湖南长沙410208

出　　处：《湖南中医药大学学报》2010年第9期90-92,101,共4页Journal of Hunan University of Chinese Medicine

基　　金：国家十一五支撑计划(2006BAI09B05-9)

摘　　要：目的通过建立HPLC指纹图谱,对金银花与山银花的化学成分进行比较研究。方法 Kromasil C18色谱柱(4.6 mm×250 mm,5μm),乙腈-0.4%的磷酸水溶液梯度洗脱,检测波长:238 nm,柱温:30℃,流速:1 mL/min。结果建立了金银花与山银花的高效液相指纹图谱,发现成分的相对含量有明显差异。结论利用HPLC指纹图谱可较全面的反映金银花与山银花间的差异。Objective To compare the Lonicera Japonica and Lonicera confusa DC.by establishing HPLC fingerprint.Method The sample was performed on a Kromasil C18（4.6 mm × 250 mm,5 μm）,acetonitril（A）-0.4% H3PO4（B） was used as mobile phase with gradient elution,the flow rate,column temperature and detection wavelength 1.0 mL/min,30 ℃ and 238 nm.Result The HPLC-fingerprint profiles of Lonicera Japonica and Lonicera confusa DC.were established,found their contents of samples were different significantly.Conclusion HPLC fingerprint profiles can reflex the difference between Lonicera Japonica and Lonicera confusa DC.

关 键 词：金银花 山银花 绿原酸 木犀草苷 HPLC指纹图谱 

分 类 号：R285.5[医药卫生—中药学]