甲氧虫酰肼对舞毒蛾解毒酶和保护酶活性的影响  

Effects of Methoxyfenozide(RH-2485) on the Activities of Detoxifying Enzymes and Protective Enzymes in Lymantria dispar(Lepidoptera:Lymantriidae)

作　　者：鄢杰明[1] 廖月枝[1] 严善春[1] 曹传旺[1] 冯春富[1] 

机构地区：[1]东北林业大学,哈尔滨150040

出　　处：《东北林业大学学报》2010年第11期112-114,共3页Journal of Northeast Forestry University

基　　金：国家公益性行业科研专项(200904029);教育部国家科技基础条件平台建设专项(2005DK21105)

摘　　要：为研究甲氧虫酰肼(RH-2485)的杀虫活性及作用机理,采用叶片药膜法测定了甲氧虫酰肼对舞毒蛾5龄幼虫的生物活性及对其体内解毒酶和保护酶活性的影响。结果表明:甲氧虫酰肼对舞毒蛾5龄幼虫表现出较高活性,48hLC50为24.271mg·L-1。48hLC50剂量甲氧虫酰肼处理24和48h后,对舞毒蛾5龄幼虫羧酸酯酶(CarE)、谷胱甘肽-S-转移酶(GST)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性均显著抑制;对多功能氧化酶(MFO)作用表现为先抑制后激活,且与对照间差异极显著(p<0.01);对酚氧化酶(PO)具有激活作用,其中处理24h,PO活性显著高于对照(p<0.05)。A study was conducted to determine the insecticidal activity and mechanism of Methoxyfenozide（RH-2485）,bioactivity of RH-2485 against the 5th instar larvae of Lymantria dispar and the effects of RH-2485 on the activities of detoxifying enzymes and protective enzymes.Results showed that Methoxyfenozide had a high level of toxicity against the fifth instar larvae and 48-h LC50 of Methoxyfenozide was 24.271 mg·L-1.The activities of carboxylesterase（CarE）,glutathione S-transferase（GST）,superoxide dismutase（SOD）,catalase（CAT） and peroxidase（POD） of the fifth instar larvae were significantly inhibited after they were fed with the leaves treated by 48-h LC50 of RH-2485 for 24 h and 48 h.However,the activity of mixed-function oxidases（MFO） was inhibited at 24 h time point and then was activated at 48 h time point after treatment by RH-2485.The activity of phenoloxidase（PO） was activated and significantly higher than that of the control at 24 h time point（P 0.05）.

关 键 词：甲氧虫酰肼 舞毒蛾 生物活性 解毒酶 保护酶 

分 类 号：Q965.9[生物学—昆虫学]