丙酮酸乙酯对大鼠全脑缺血再灌注损伤海马区SOD、NO和iNOS的影响  

Effects of ethyl pyruvate on SOD,NO,iNOS of hippocampal regions in rats with global cerebral ischemia reperfusion injury

作　　者：徐卫财[1] 倪玉霞[1] 刘小青[1] 江朝秀[1] 

机构地区：[1]广西医科大学第一附属医院麻醉科,广西南宁530021

出　　处：《中国现代医学杂志》2010年第1期45-48,共4页China Journal of Modern Medicine

基　　金：广西自然科学基金项目(No:桂科自0640091)

摘　　要：目的探讨丙酮酸乙酯对大鼠全脑缺血再灌注损伤大脑海马区组织细胞因子SOD、NO、iNOS表达的影响,为进一步的研究提供依据。方法该实验采用二血管阻断加低血压法制作大鼠全脑缺血再灌注模型。SD大鼠24只,随机被分成3组,每组8只:①假手术组(F组):只分离股动脉和双侧颈总动脉,不降压,不夹闭双侧颈总动脉;②缺血再灌注组(IR组):股动脉放血使血压降至基础血压的50～60%,同时夹闭双侧颈总动脉10min再开放;③丙酮酸乙酯处理组(EP组):与IR组处理相同。EP组于开放恢复血流即刻腹腔注射丙酮酸乙酯40mg/kg,其余2组在同一时刻给予等量生理盐水。再灌注后6h断头取脑,测定海马区组织SOD、NO含量和iNOS活性。同时,制作光镜标本,在光镜下行海马区形态学和病理组织学观察。结果IR组SOD、NO、i-NOS的表达水平均高于F组,差异有统计学意义(P<0.05);与IR组比较,EP组SOD的表达增加,而NO、i-NOS的表达水平下降,差异有统计学意义(P<0.05);光镜形态学图片中,IR组海马区细胞损伤数明显高于F组,差异有统计学意义(P<0.05);EP组细胞损伤数较IR组下降,差异有统计学意义(P<0.05)。结论丙酮酸乙酯可能通过减轻氧化应激反应,对大鼠全脑缺血再灌注细胞损伤起保护作用。【Objective】To investigate the effects of ethyl puruvate on expression of SOD,NO,iNOS of hippocampal regions in rats with global cerebral ischemia reperfusion injury and provide the evidences for further study. 【Methods】The models were made by transient bilateral occlusion of the common carotid artery （CCA） and hypotension lasting for 10 min according to the method of Smith. Twenty-four male Sprague-Dawley （SD） rats weighing 300～400 g were randomly divided into 3 groups （n=8,respectively）：Control group （F group）,only exposed three arteries. ②Ischemia reperfusion group （IR group）,bloodletting （lowered the blood pressure to 50～60% of base value） and transient occlusion of the bilateral common carotid arteries for 10 min. Ethyl pyruvate theraphy group （EP group）,the same technique as IR group. EP （40 mg/kg） was immediately administered in EP group by intraperitoneal injection after ischemia-reperfusion. Saline water was administered in the other two groups by the same procedures. 6 hours later,the rats were sacrificed and brain tissues were isolated for the test of SOD,NO,iNOS factors by color matching method and for histopathological examination （Light microscope）.【Results】The levels of SOD,NO,iNOS,in IR group were higher than those in F group （P〈0.05）. But compared with IR group,the levels of SOD were increased and NO,iNOS in EP group were decreased （P〈0.05）. The number of injury cells （Light microscope） in IR group was obviously increased compared with F group,but decreased in EP group.【Conclusion】Ethyl Pyruvate can defend the brain ischemia reperfusion injury by alleviating the oxidative stress reaction.

关 键 词：大脑缺血再灌注 丙酮酸乙酯 SOD NO INOS 

分 类 号：R605.97[医药卫生—急诊医学]