己酮可可碱对染汞大鼠脊髓及背根神经节TNF-α表达的影响  

Effect of pentoxifylline on TNF-a expression in spinal cord and DRG of mercurialism rat

作　　者：张军 周永田 徐旭东 谢林 李娟 徐明怡 

机构地区：[1]山东省医药工业设计院,山东济南250013 [2]山东省职业卫生和职业医学院,山东济南250002

出　　处：《中国现代医学杂志》2010年第4期501-504,共4页China Journal of Modern Medicine

基　　金：山东省医学科学院资助项目(2004-0023)

摘　　要：目的观察己酮可可碱对大鼠脊髓和背根神经节(DRG)肿瘤坏死因子-α(TNF-α)的影响。方法雄性SD大鼠40只,体重180～220g,分为7组。对照组(10只)经口灌胃生理盐水(NS)2mL/次,每天1次;A、B、C组(各5只)按17mg/kg;D、E、F组(各5只)按8.5mg/kg经口灌胃HgCl2溶液,每天1次。7组均连续灌胃18～22d。染汞组动物出现汞中毒症状后,处死A、D组大鼠和对照组5只大鼠。随后B、E组用二巯丙磺钠注射液(DMPS)按28mg/kg腹腔注射驱汞2个疗程(驱汞3d,休息4d为1个疗程);C组用DMPS+己酮可可碱(按29.22mg/kg经口灌胃,每天1次,共14d);F组腹腔注射NS1mL/次,方法同DMPS。实验结束后处死全部动物,灌注固定取出脊髓L5-6节及L5、L6两侧DRG并制成病理切片。用免疫组织化学SABC法测定DRG和脊髓TNF-α表达水平。结果染毒组大鼠DRG和脊髓TNF-α平均灰度均显著低于对照组;阳性反应物平均面积均显著高于对照组(P<0.05);B、E组用DMPS驱汞2个疗程及F组腹腔注射NS2周期后,均未见TNF-α表达显著减弱,C组(DMPS+己酮可可碱)DRG和脊髓TNF-α平均灰度显著提高,平均面积减少(P<0.05)。结论亚急性HgCl2中毒大鼠DRG和脊髓TNF-α表达显著增强,己酮可可碱联合DMPS能够有效抑制TNF-α表达。【Objective】To investigate the effect of pentoxifylline on tumor necrosis factor-α （TNF-α） in spinal cord and dorsal root ganglion （DRG）. 【Methods】Forty SD rats （male, weighting 180-220 g） were divided into 7 groups. Group A, B and C （5 rats in each group） received HgCl2 juice at dose of 17 mg/kg （1/4 LD50）, group D, E and F （5 rats in each group） received HgCl2 juice at dose of 8.5 mg/kg （1/8 LD50） by gavage daily. Control group （10 rats） received normal saline （NS） 2 mL by gavage daily. The sub-acute mercury poisoning rat models were established within （20±2） days. Then five rats in each of control group, group A and group D were dissected and the chelator （DMPS） was injected into the abdomen of the alive rats of group B and E at dose of 28 mg/kg for 2 periods. Group C was given both DMPS and pentoxifylline and group F was injected 1 mL NS into the abdomen of rats only. The pentoxifylline was used at dose of 29.22 mg/kg by gavage daily for 14 days. The rats were all dissected and the spinal cord （L5-6） and the DRG of L5 and L6 were gotten after the trial was finished, and the pathological sections were made. TNF-α was detected according to immunohistochemistry SABC way. 【Results】The mean gray scale value of TNF-αin spinal cord and DRG of the rats exposed to HgCl2 was decreased significantly compared with that of control group （P 0.05）. They are not decreased significantly after two 7-day cycles for group F using NS and groups B and E using DMPS compared with control group. The mean gray scale value of TNF-α in spinal cord and DRG of group C was increased significantly after 2 periods of DMPS and pentoxifylline administration compaired with that of group B （P 0.05）.【Conclusion】 TNFαlevels in spinal cord and DRG of sub-acute mercurialism rats are increased significantly compaired with that of controls and they are inhibited significantly by pentoxifylline combined with DMPS.

关 键 词：己酮可可碱 汞中毒 神经病理性疼痛 肿瘤坏死因子-A 脊髓 背根神经节 

分 类 号：R135.13[医药卫生—劳动卫生]