紫杉醇对人绒癌细胞增殖及凋亡的影响  

Effect of paclitaxel on proliferation and apoptosis of human choriocarcinoma cell line JEG-3

作　　者：曹兰琴[1] 黎欣[1] 刘惠宁[1] 易红[1] 李岑[1] 

机构地区：[1]中南大学湘雅医院妇产科,湖南长沙410008

出　　处：《中国现代医学杂志》2010年第4期510-514,共5页China Journal of Modern Medicine

摘　　要：目的研究紫杉醇(Paclitaxel)对绒癌细胞株JEG-3细胞凋亡率和细胞周期的影响。方法用紫杉醇作用于绒癌细胞株JEG-3细胞,采用四甲基偶氮唑蓝(MTT)比色法观察紫杉醇对绒癌细胞增殖的影响,通过流式细胞仪分析紫杉醇处理后绒癌细胞凋亡率和细胞周期的改变。结果紫杉醇对绒癌细胞株JEG-3细胞的增殖具有抑制作用,且随药物浓度的增加和作用时间的延长,抑制作用越明显;紫杉醇对细胞凋亡率和细胞周期均有影响,随药物浓度增加和作用时间的延长,细胞凋亡率增加,G2/M期细胞百分率增加。结论紫杉醇能有效诱导绒癌JEG-3细胞的凋亡。【Objective】To explore apoptosis and cell cycle distribution of human choriocarcinoma cell line JEG-3 treated by paclitaxel. 【Methods】The effect of paclitaxel on the proliferation of human choriocarcinoma cell line JEG-3 was evaluated by MTT growth assay. The rate of apoptosis and the cell cycle distribution were analyzed by flow cytometry. 【Results】Paclitaxel could inhibit the proliferation of cell line JEG-3. The inhibition effects were concentration and time dependent. Paclitaxel could affect the apoptosis of cells and the cell cycle distribution. The rate of apoptosis and the percentage of G2/M cells increased as the concentration of paclitaxel increased and the action time was prolonged.【Conclusion】Paclitaxel can induce apoptosis of human choriocarcinoma cell line JEG-3.

关 键 词：紫杉醇 绒毛膜癌 凋亡 细胞周期 

分 类 号：R271.9[医药卫生—中西医结合] R730.5[医药卫生—中医妇科学]