脂联素基因多态性与2型糖尿病的相关性研究(英文)  

Correlation between adiponectin gene polymorphisms and type 2 diabetes

作　　者：李奕平[1] 姚宇峰[2] 张瑛[1] 李显丽[1] 陶文玉[1] 徐凡[1] 王飞英[1] 杨曼[1] 

机构地区：[1]云南省第二人民医院内分泌代谢科,云南昆明650021 [2]中国医学科学院(北京协和医学院)医学生物学研究所,云南昆明650118

出　　处：《中国现代医学杂志》2010年第7期961-965,970,共6页China Journal of Modern Medicine

基　　金：supported by the Education Department Fund of Yunnan Province(No:09C0296)

摘　　要：目的探讨脂联素基因多态性与2型糖尿病的相关性。方法本研究入选昆明地区汉族2型糖尿病患者133名,否认糖尿病家族史的非糖尿病个体57名。采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测脂联素基因SNP-11377、SNP+45和SNP+276基因型,构建单倍型。评估以上三个多态性位点与2型糖尿病的相关性。结果①脂联素基因SNP-11377、SNP+45和SNP+276的基因型和等位基因频率在昆明地区汉族2型糖尿病组和非2型糖尿病组中无统计学差异。②SNP-11377、SNP+45和SNP+276所构建的单倍型频率在昆明地区汉族2型糖尿病组和非2型糖尿病组中无统计学差异。③在非2型糖尿病组中,SNP-11377CC基因型个体的体重指数和腰围较低。结论脂联素基因SNP-11377、SNP+45和SNP+276与昆明地区汉族2型糖尿病无相关性。SNP-11377CC基因型可能是昆明地区汉族非糖尿病个体肥胖的一个保护基因型。【Objective】To investigate the correlation between adiponectin gene polymorphisms and type 2 diabetes（T2DM）.【Methods】Totally 133 patients with type 2 diabetes and 57 nondiabetes（NDM） subjects with no family history of diabetes in Kunming,Yunnan Province,Southwest of China,were included in the present study.Using polymerase chain reaction-based restriction fragment length polymorphism（PCR-RFLP）,the genotypes of SNP-11377,SNP＋45 and SNP＋276 in adiponectin gene were detected and their haplotypes were constructed.Then,the correlation between the three SNPs and T2DM of Han population was assessed.【Results】 The genotype and allele frequencies of SNP-11377,SNP ＋45 and SNP ＋276 had no significant difference between T2DM and NDM groups.The constructed haplotype frequencies of SNP-11377,SNP＋45 and SNP＋276 had no significant difference between T2DM and NDM groups.In NDM group,subjects with SNP-11377CC genotype had lower body mass index（BMI） and waist circumference.【Conclusions】There is no correlation between the SNP-11377,SNP＋45,SNP＋276 and T2DM of Han population in Kunming.SNP-11377CC genotype might be a protective genotype against obesity in NDM group of Han population in Kunming.

关 键 词：脂联素基因 单核苷酸多态性 2型糖尿病 

分 类 号：R587.1[医药卫生—内分泌]