HSP70通过抑制NF-κB信号通路促进大肠杆菌诱导的人巨噬细胞系U937细胞凋亡(英文)  

HSP70 promotes E.coli-mediated U937 cell apoptosis by inhibiting NF-κB signaling pathway

作　　者：阚亮[1] 王佳贺[1] 周一军[2] 何平[1] 

机构地区：[1]中国医科大学附属盛京医院老年病科,辽宁沈阳110004 [2]中国医科大学附属第四医院内分泌科,辽宁沈阳110032

出　　处：《中国现代医学杂志》2010年第7期966-970,共5页China Journal of Modern Medicine

基　　金：Supported by the doctor research start-up fund of Liaoning province(20081055);a grant from the education department of Liaoning province(2009A737)

摘　　要：目的探讨大肠杆菌(E.coli)感染与人巨噬细胞系U937细胞凋亡的关系及热休克蛋白70(HSP70)、核因子(nuclear factor kappa B,NF-κB)表达的变化。方法以Annexin V FITC/PI双染流式细胞仪检测为指标,研究E.coli感染对人巨噬细胞系U937细胞凋亡的诱导作用;用Western blot方法检测HSP70和NF-κB的表达。结果Annexin V FITC/PI双染结果发现,U937细胞凋亡百分率随E.coli浓度的增加而增加,HSP70的表达随着E.coli浓度的增加逐渐升高,而NF-κB的表达逐渐降低。结论E.coli诱导U937细胞凋亡过程可能与HSP70表达的升高和NF-κB表达的降低有关。【Objective】To investigate the relationship between apoptosis of human monocytic U937 cells and their expression of heat shock protein 70 kd（HSP70） and nuclear factor kappa B（NF-κB） induced by Escherichia coli（E.coli）.【Methods】Cell apoptosis was analyzed by Annexin V FITC/PI assay.Western blot was used to detect the expressions of HSP70 and NF-κB protein.【Results】Annexin V FITC/PI assay revealed that the percentage of apoptotic cells rose with the increasing concentrations of E.coli.Also,there was a tendency for HSP70 protein expression to increase and for NF-κB protein expression to decrease with the increased E.coli concentrations.【Conclusions】U937 cell apoptosis induced by E.coli may be related to the increase of HSP70 expression and decrease of NF-κB expression.

关 键 词：大肠杆菌 细胞凋亡 U937 NF-ΚB HSP70 

分 类 号：R378.2[医药卫生—病原生物学]